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Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2
Polyclonal antibody raised in rabbit against the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.
Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO Figure 2A: Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel. Figure 2B: Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. Figure 2C: Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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