AML-ETO polyclonal antibody

Catalog Number
50 µg/250 µl
  Bulk order

Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2

Polyclonal antibody raised in rabbit against the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.

Concentration0.2 µg/µl
Species reactivityHuman, mouse
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 Fig 1
Western Blotting 1:1,000 Fig 2
  • Validation Data


    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO
    Figure 2A:  Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.
    Figure 2B: Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein.
    Figure 2C: Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.

  •  実験手法
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  資料
    Datasheet AML-ETO pAb-080-050 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: AML-ETO polyclonal antibody (Diagenode Cat# C15410080 Lot# A134-0011). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Esculetin Downregulates the Expression of AML1-ETO and C-Kit in Kasumi-1 Cell Line by Decreasing Half-Life of mRNA
    Sawney S, Arora R, Aggarwal KK, Saluja D
    One of the most frequent genetic aberrations in acute myeloid leukemia (AML) is chromosomal translocation between AML1/RUNX1 on chromosome 21 and ETO gene on chromosome 8 resulting in the expression of chimeric oncogene AML1-ETO. Although patients with t(8;21) translocation have good prognosis, 5-year survival is ob...

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