Application Note: Combining the use of the iDeal® CUT&Tag kit and the Tissue Nuclei Isolation Module for chromatin profiling in iPSC-derived cortical brain organoids. The iDeal CUT&Tag kit has been designed for efficient chromatin profiling of histone marks and some non-histone proteins. CUT&Tag is a recently developed strategy to profile the location of...
Application Note: Mapping Histone post-translational modifications in the Pacific oyster using iDeal CUT&Tag and the Tissue Nuclei Extraction Module. Mollusks represent one of the largest and most diverse phyla in the animal kingdom (Escoubas et al., 2016). Nevertheless, the different carriers of epigenetic information have only rarely been stud...
Application note: Best Workflow Practices for ChIP-seq Analysis with Small Samples Combined chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) has become the gold standard to investigate genome-wide epigenetic profiles. However, ChIP from a limited amou...
Chromatin shearing with the Diagenode One The Diagenode One is a new affordable solution for Chromatin shearing in ChIP analysis for small samples. Designed to fit any bench, it is the smallest and lightest Diagenode shearing de...
Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation of most archived pathological specimens. Archival FFPE samples provide an invaluable repository of in...
LC-MS measurement of whole-body uptake of pharmaceuticals in zebrafish using the Bioruptor® Plus for sample preparation The laboratory for Pharmaceutical Analysis at KU Leuven is developing innovative separation and detection techniques in pharmaceutical applications. Within the Department of Pharmaceutical and Phar...
Optimal long DNA fragment generation prior to SMRTbell® library preparation Several methods exist today for shearing genomic DNA, but few are reliable for generating DNA fragments >50 kb necessary for assembly of many microbial, human, plant and animal genomes de novo. ...
Efficient and streamlined solution for high-quality NGS library preparation using the Bioruptor® Pico and JetSeqTM Flex DNA Library Preparation Kit Next Generation Sequencing workflows include a number of critical steps which can have a significant impact both on the quality of the results and on the overall cost of the project. In particular,...
Comparison of the S. cerevisae chromatin shearing with Covaris™ S2 and Bioruptor® Pico The chromatin shearing is very important step for all kind of ChIP analysis followed by next-generation sequencing. The efficiency, reproducibility and straightforwardness are the most important ch...
Ultrasensitive proteomics for microdissected fresh frozen tissue samples This protocol, specifically designed for ultrasensitive proteomics, enables high throughput quantitative proteomics study on microdissected tissue samples. The Bioruptor® Pico provided high eff...
Optimize the selection of guide RNA by ChIP to keep CRISPR on-target The mechanisms of target recognition and target specificity of the Cas9 protein is still not completely understood. A major hurdle of this technology is the introduction of double-strand breaks (DS...
Sequence of the methylated spike-in control in FASTA format This document and files are to process the sequencing data from the spike-in controls included in the RRBS kit in order to estimate the bisulfite conversion efficiency. You can find the sequences o...
Sequence of the unmethylated spike-in control in FASTA format This document and files are to process the sequencing data from the spike-in controls included in the RRBS kit in order to estimate the bisulfite conversion efficiency. You can find the sequences o...
Positions of the unmethylated cytosines in the sequence of the methylated control in BED format This document and files are to process the sequencing data from the spike-in controls included in the RRBS kit in order to estimate the bisulfite conversion efficiency. You can find the sequences o...
acrIIA2 monoclonal antibody datasheet Monoclonal antibody raised in mouse against acrIIA2 (anti CRISPR protein IIA2) using the full length recombinant protein.
ChIP workshop 08/12/2020
ChIP workshop 16-17/02/2021
Compare Bioruptor Features (Tables)
Diagenode Bioruptor DNA QC kit analysis template
ChIP workshop 15/06/2021
