Diagenode

SMAD3 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410328
100 μg/ 86 μl
$295.00
  Bulk order

Alternative names: MADH3, JV15-2, HMAD-3, HSMAD3, MAD3, LDS3, HSPC193, SMAD 3

Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) using a KLH conjugated synthetic peptide from the C-terminal par of the protein.

Lot001
Concentration1.17 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:500 Fig 1
Immunohistochemistry 1:400 Fig 2
  • Validation data

    WB

    Figure 1. Western blot analysis using the Diagenode antibody directed against SMAD3
    Lysates from different cells treated with TGFb (+) or untreated control cells (-) were analysed by Western blot using the Diagenode antibody against SMAD3 (Cat. No. C15410328) diluted 1:500. The position of SMAD3 is indicated on the right. The antibody recognizes both the phopsphorylated and the unphosphorykated SMAD3.

    IHC

    Figure 2. Immunohistochemistry using the Diagenode antibody directed against SMAD3
    Formalin fixed paraffin embedded cancerous human liver tissue was stained with the Diagenode antibody against SMAD3 (Cat. No. C15410328) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. The presence of the SMAD3 protein is shown by the grey color; red indictaed the nuclei.

  • Target description

    SMAD3 (UniProt/Swiss-Prot entry P84022) belongs to the SMAD (‘mothers against decapentaplegic) family of proteins which are signal transducers and transcriptional modulators involved in multiple pathways. SMAD3 is activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases which phosphorylates the C-terminal serines 423 and 425. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and is involved in chondrogenesis, osteogenesis and wound healing. It is also thought to play a role in carcinogenesis.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IHC
    Immunohistochemistry Read more
  • Documents
    SMAD3 polyclonal antibody C15410328 DATASHEET
    Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) using a KLH conjugated ...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SMAD3 polyclonal antibody - Classic (Diagenode Cat# C15410328 Lot# 001). Click here to copy to clipboard.

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