Diagenode

NRF1 monoclonal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200013
50 μg/23 μl
$295.00
  Bulk order

Monoclonal antibody raised in mouse against human NRF1 (Nuclear Respiratory Factor 1), using a recombinant protein. 

Lot001
Concentration2.2 μg/μl
Species reactivityHuman
TypeMonoclonal
PurityProtein A purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2 μg/ChIP Fig 1, 2
Western Blotting 1:1,000 Fig 3

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.

  • Validation data

    ChIP figure 1

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1
    ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq figure 2

    ChIP-seq figure 2

    ChIP-seq figure 2

    ChIP-seq figure 2

    Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1
    ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).

    Western blot figure 3

    Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1
    Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Applications
    ChIP-qPCR (ab)
    Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    NRF1 monoclonal antibody DATASHEET
    Datasheet of the NRF1 monoclonal antibody
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: NRF1 monoclonal antibody - Classic (Diagenode Cat# C15200013 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

Events

  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
  • XXXIX Reunión anual de la Sociedad de Bioquimica y Biología Molecular de Chile
    Puerto Varas, Chile
    Sep 27-Sep 30, 2016
 See all events

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