Diagenode

NFYA polyclonal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310261
100 μl
$295.00
  Bulk order

Alternative names: NF-YA, HAP2, CBF-A, CBF-B

Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), using a KLH conjugated synthetic peptide from the N-terminus of the protein.

Lot001
Concentrationnot determined
Species reactivityHuman
TypePolyclonal ChIP grade, ChIP-seq grade
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1:1,000 Fig 1, 2
Western Blotting 1:1,000 Fig 3
Immunohistochemistry 1:400 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA
    ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A.ChIP-seq
    B.ChIP-seq
    C.ChIP-seq
    D.ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA
    ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.

    WB

    Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA
    Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.

    IF

    Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA
    Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).

  • Target description

    NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IHC
    Immunohistochemistry Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    NFYA polyclonal antibody C15310261 DATASHEET
    Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), us...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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  • Publications

    How to properly cite this product in your work

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