Diagenode

CRISPR/Cas9 C-terminal monoclonal antibody (sample size)

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200223-14
14 µg
$99.00
  Bulk order
Other format

Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9)

Lot001
Concentration2.2 µg/µl
Species reactivityStreptococcus pyogenes
TypeMonoclonal
PurityProtein G purified
HostMouse
StorageStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 - 1:5,000 Fig 1,2
IP 13 μg/IP Fig 3
IF 1:200 Fig 4
  • Validation data

    Western Blot

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9
    Western blot was performed on protein extracts from HeLa cells transfected with Cas9 using the Diagenode antibody against CRISPR/Cas9 (cat. No. C15200223). The antibody was used at different dilutions. The marker is shown on the left, position of the Cas9 protein is indicated on the right.

    Western Blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9
    Western blot was performed on 20 μg protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9 (cat. No. C15200223). The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right.

    IP

    Figure 3. IP using the Diagenode monoclonal antibody directed against Cas9
    IP was performed on whole cell extracts (250 μg) from HeLa cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 13 μg of the Diagenode antibody against Cas9 (cat. No. C15200223). The immunoprecipitated proteins were subsequently analysed by Western blot. Lane 3 and 4 show the result of the IP, the input (15 μg) is shown in lane 1 and 2.

    IP

    Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
    HeLa cells expressing Cas9 under the control of the tight TRE promoter were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (cat. No. C15200223) diluted 1:200, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488. Nuclei were counter-stained with Hoechst 33342. Figure 4 shows the result in the presence (left) or absence (right) of doxycycline.

  • Background

    A breakthrough in genome engineering! The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IP
    Immunoprecipitation Read more
  • Documents
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CRISPR/Cas9 C-terminal monoclonal antibody (sample size) (Diagenode Cat# C15200223-14 Lot# 001). Click here to copy to clipboard.

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