Diagenode

Blue ladder monoclonal antibody

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200213
50 μl
$85.00
  Bulk order

Monoclonal antibody raised in mouse against the blue colour used in many prestained protein MW markers. This antibody can be used to visualize the marker bands after WB.

Lot003D
Concentration2 μg/μl
Species reactivity
TypeMonoclonal
PurityProtein A purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 - 1:5,000 Fig 1
  • Validation data

    WB figure 1

    Figure 1. Western blot analysis using the Diagenode Blue ladder monoclonal antibody
    5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder antibody (Cat. No. C1520213) used at different dilutions. Figure 1 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Blue ladder monoclonal antibody C15200213 DATASHEET
    Tds of the Blue ladder monoclonal antibody
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Blue ladder monoclonal antibody (Diagenode Cat# C15200213 Lot# 003D). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Anti-RAINBOW dye-specific antibodies as universal tools for the visualization of prestained protein molecular weight markers in Western blot analysis
    Schüchner S et al.
    Western blotting is one of the most widely used techniques in molecular biology and biochemistry. Prestained proteins are used as molecular weight standards in protein electrophoresis. In the chemiluminescent Western blot analysis, however, these colored protein markers are invisible leaving researchers with the uns...

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