Comprehensive characterization of the epigenetic landscape in Multiple Myeloma Published: January, 2022 Abstract:
Background: Human multiple myeloma (MM) cell lines (HMCLs) have been widely used to understand themolecular processes that drive MM biology. Epigenetic modifications are involved in MM development,progression, and drug resistance. A comprehensive ... Read more »
Genome-wide redistribution of BRD4 binding sites in transformation resistant cells. Published: March, 2015 Abstract:
Hutchinson-Gilford progeria syndrome (HGPS) patients do not develop cancer despite a significant accumulation of DNA damage in their cells. We have recently reported that HGPS cells are refractory to experimental oncogenic transformation and we id... Read more »
Recurrent ETNK1 mutations in atypical chronic myeloid leukemia. Published: January, 2015 Abstract:
Despite the recent identification of recurrent SETBP1 mutations in atypical chronic myeloid leukemia (aCML), a complete description of the somatic lesions responsible for the onset of this disorder is still lacking. To find additional somatic abno... Read more »
Neuropeptide Y promotes adipogenesis in chicken adipose cells in vitro. Published: November, 2014 Abstract:
Neuropeptide Y is an evolutionarily conserved neurotransmitter that stimulates food intake in higher vertebrate species and promotes adipogenesis in mammals. The objective of this study was to determine if NPY also enhances adipogenesis in birds, ... Read more »
DNA Copy-Number Control through Inhibition of Replication Fork Progression. Published: November, 2014 Abstract:
Proper control of DNA replication is essential to ensure faithful transmission of genetic material and prevent chromosomal aberrations that can drive cancer progression and developmental disorders. DNA replication is regulated primarily at the lev... Read more »
Genome-wide map of regulatory interactions in the human genome. Published: September, 2014 Abstract:
Increasing evidence suggests that interactions between regulatory genomic elements play an important role in regulating gene expression. We generated a genome-wide interaction map of regulatory elements in human cells (ENCODE tier 1 cells, K562, G... Read more »
Hypomethylated pollen bypasses the interploidy hybridization barrier in Arabidopsis. Published: September, 2014 Abstract:
Plants of different ploidy levels are separated by a strong postzygotic hybridization barrier that is established in the endosperm. Deregulated parent-of-origin specific genes cause the response to interploidy hybridizations, revealing an epigenet... Read more »
RPLP1, a Crucial Ribosomal Protein for Embryonic Development of the Nervous System. Published: June, 2014 Abstract:
Ribosomal proteins are pivotal to development and tissue homeostasis. RP Large P1 (Rplp1) overexpression is associated with tumorigenesis. However, the physiological function of Rplp1 in mammalian development remains unknown. In this study, we dis... Read more »
Early aberrant DNA methylation events in a mouse model of acute myeloid leukemia. Published: April, 2014 Abstract:
BACKGROUND: Aberrant DNA methylation is frequently found in human malignancies including acute myeloid leukemia (AML). While most studies focus on later disease stages, the onset of aberrant DNA methylation events and their dynamics during leukemi... Read more »
Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1. Published: April, 2014 Abstract:
Genetic deficiencies in transcription factors can lead to the loss of certain types of cells and tissue. The steroidogenic tissue-specific nuclear receptor Ad4BP/SF-1 (NR5A1) is one such gene, because mice in which this gene is disrupted fail to d... Read more »
A deterministic analysis of genome integrity during neoplastic growth in Drosophila. Published: February, 2014 Abstract:
The development of cancer has been associated with the gradual acquisition of genetic alterations leading to a progressive increase in malignancy. In various cancer types this process is enabled and accelerated by genome instability. While genome ... Read more »
CLOCK:BMAL1 is a pioneer-like transcription factor. Published: January, 2014 Abstract:
The mammalian circadian clock relies on the master genes CLOCK and BMAL1 to drive rhythmic gene expression and regulate biological functions under circadian control. Here we show that rhythmic CLOCK:BMAL1 DNA binding promotes rhythmic chromatin op... Read more »
Inactivating CUX1 mutations promote tumorigenesis. Published: January, 2014 Abstract:
A major challenge in cancer genetics is to determine which low-frequency somatic mutations are drivers of tumorigenesis. Here we interrogate the genomes of 7,651 diverse human cancers and find inactivating mutations in the homeodomain transcriptio... Read more »
Genome-wide chromatin analysis in mature mouse and human spermatozoa. Published: December, 2013 Abstract:
At the end of mammalian spermatogenesis, chromatin in differentiating germ cells is extensively remodeled, with the majority of nucleosomes being removed and ultimately exchanged by highly basic proteins named protamines. Residual nucleosomes are,... Read more »
The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana. Published: October, 2013 Abstract:
How alternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) is involved in AS in Arabidopsis thaliana. Here we show that both subunits of AtCBC (A... Read more »
Gene regulation and priming by topoisomerase IIα in embryonic stem cells. Published: September, 2013 Abstract:
Topoisomerases resolve torsional stress, while their function in gene regulation, especially during cellular differentiation, remains unknown. Here we find that the expression of topo II isoforms, topoisomerase IIα and topoisomerase IIβ, is the ch... Read more »
The BTB-Containing Protein Kctd15 Is SUMOylated In Vivo. Published: September, 2013 Abstract:
Potassium Channel Tetramerization Domain containing 15 (Kctd15) has a role in regulating the neural crest (NC) domain in the embryo. Kctd15 inhibits NC induction by antagonizing Wnt signaling and by interaction with the transcription factor AP-2α ... Read more »
MeCP2 modulates gene expression pathways in astrocytes. Published: January, 2013 Abstract:
ABSTRACT: BACKGROUND: Mutations in MECP2 encoding methyl-CpG-binding protein 2 (MeCP2) cause the X-linked neurodevelopmental disorder Rett syndrome. Rett syndrome patients exhibit neurological symptoms that include irregular breathing, impaired mo... Read more »
Evolution of cichlid vision via trans-regulatory divergence. Published: December, 2012 Abstract:
ABSTRACT: BACKGROUND: Phenotypic evolution may occur through mutations that affect either the structure or expression of protein-coding genes. Although the evolution of color vision has historically been attributed to structural mutations within t... Read more »
A polycomb group protein is retained at specific sites on chromatin in mitosis. Published: December, 2012 Abstract:
Epigenetic regulation of gene expression, including by Polycomb Group (PcG) proteins, may depend on heritable chromatin states, but how these states can be propagated through mitosis is unclear. Using immunofluorescence and biochemical fractionati... Read more »
Systematic identification of functional residues in mammalian histone H2AX. Published: October, 2012 Abstract:
The histone variant H2AX is a principal component of chromatin involved in the detection, signaling and repair of DNA double-strand breaks (DSBs). H2AX is thought to operate primarily through its C-terminal S139 phosphorylation, which mediates the... Read more »
PML promotes MHC class II gene expression by stabilizing the class II transactivator. Published: September, 2012 Abstract:
Promyelocytic leukemia (PML) nuclear bodies selectively associate with transcriptionally active genomic regions, including the gene-rich major histocompatibility (MHC) locus. In this paper, we have explored potential links between PML and interfer... Read more »
Transcriptional networks driving enhancer function in the CFTR gene. Published: June, 2012 Abstract:
A critical cis-regulatory element for the cystic fibrosis transmembrane conductance regulator (CFTR) gene is located in intron 11, 100 kb distal to the promoter, with which it interacts. This sequence contains an intestine-selective enhancer and a... Read more »
Miz1 Is a Critical Repressor of cdkn1a during Skin Tumorigenesis. Published: April, 2012 Abstract:
The transcription factor Miz1 forms repressive DNA-binding complexes with the Myc, Gfi-1 and Bcl-6 oncoproteins. Known target genes of these complexes encode the cyclin-dependent kinase inhibitors (CKIs) cdkn2b (p15(Ink4)), cdkn1a (p21(Cip1)), and... Read more »
Dynamic MicroRNA Gene Transcription and Processing during T Cell Development. Published: February, 2012 Abstract:
By disrupting microRNA (miRNA) biogenesis, we previously showed that this pathway is critical for the differentiation and function of T cells. Although various cloning studies have shown that many miRNAs are expressed during T cell development, an... Read more »
Osteocalcin gene expression is regulated by wild-type p53. Published: November, 2011 Abstract:
The tumor-suppressor p53 is a transcription factor that regulates a number of genes in the process of cell-cycle inhibition, apoptosis, and DNA damage. Recent studies have revealed a crucial role for p53 in bone remodeling. In our previous studies... Read more »
Epigenetic-Mediated Downregulation of μ-Protocadherin in Colorectal Tumours Abstract:
Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorect... Read more »
Step-by-step preparation of proteins for mass spectrometric analysis. Abstract:
Nowadays, identification of proteins from biological samples by mass spectrometry is widely used. In principle there are two scenarios. Proteins are pre-fractionated in some way, e.g. by gel electrophoresis or are analyzed as complex mixture (shot... Read more »
ChIP-exonuclease in mammalian cells (Prot 66) Abstract:
ChIP - exonuclease (ChIP - exo) is a high resolution method of genome - wide mapping of DNA - associated proteins that outperforms ChIP - seq by all parameters . We adap ted this method created by Fran k Pugh and Ho Sung Rhee from SOLiD to the Ill... Read more »
Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation. Abstract:
Normal DNA methylation is an epigenetic modification required for proper development. Aberrant DNA methylation, in contrast, is frequently observed in many different malignancies including leukemias and lymphomas. Global DNA methylation profiling ... Read more »
