Diagenode

Interplay of cell–cell contacts and RhoA/MRTF‐A signaling regulates cardiomyocyte identity


Dorn et al

Cell–cell and cell–matrix interactions guide organ development and homeostasis by controlling lineage specification and maintenance, but the underlying molecular principles are largely unknown. Here, we show that in human developing cardiomyocytes cell–cell contacts at the intercalated disk connect to remodeling of the actin cytoskeleton by regulating the RhoA‐ROCK signaling to maintain an active MRTF/SRF transcriptional program essential for cardiomyocyte identity. Genetic perturbation of this mechanosensory pathway activates an ectopic fat gene program during cardiomyocyte differentiation, which ultimately primes the cells to switch to the brown/beige adipocyte lineage in response to adipogenesis‐inducing signals. We also demonstrate by in vivo fate mapping and clonal analysis of cardiac progenitors that cardiac fat and a subset of cardiac muscle arise from a common precursor expressing Isl1 and Wt1 during heart development, suggesting related mechanisms of determination between the two lineages.

Tags
Bioruptor
H3K4me3 (C15410003)
Bioruptor Plus

Share this article

Published
May, 2018

Source

Products used in this publication

  • some alt
    B01020001
    Bioruptor® Plus sonication device
  • Antibody ChIP-seq grade icon
    C15410030
    H3K4me3 polyclonal antibody - Classic

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics