Diagenode

Intronic RNAs mediate EZH2 regulation of epigenetic targets.


Guil S, Soler M, Portela A, Carrère J, Fonalleras E, Gómez A, Villanueva A, Esteller M

Epigenetic deregulation at a number of genomic loci is one of the hallmarks of cancer. A role for some RNA molecules in guiding repressive polycomb complex PRC2 to specific chromatin regions has been proposed. Here we use an in vivo cross-linking method to detect and identify direct PRC2-RNA interactions in human cancer cells, revealing a number of intronic RNA sequences capable of binding to the core component EZH2 and regulating the transcriptional output of its genomic counterpart. Overexpression of EZH2-bound intronic RNA for the H3K4 methyltransferase gene SMYD3 is concomitant with an increase in EZH2 occupancy throughout the corresponding genomic fragment and is sufficient to reduce levels of the endogenous transcript and protein, resulting in reduced growth capability in cell culture and animal models. These findings reveal the role of intronic RNAs in fine-tuning gene expression regulation at the level of transcriptional control.

Tags
Antibody
LowCell ChIP kit
H3K4me3 (C15410003)
H3K27me3 (C15410069)

Share this article

Published
June, 2012

Source

Related product

  • Histone-Deacetylase-polyclonal-antibody-diagenode
    C15410069
    H3K27me3 polyclonal antibody - Classic
  • Histone-Deacetylase-polyclonal-antibody-diagenode
    C15410003-50
    H3K4me3 polyclonal antibody - Premium

Events

 See all events

Twitter feed

News

 See all news