Diagenode

Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing.


Alonge Michael and Lebeigle Ludivine and Kirsche Melanie and Jenike Katie and Ou Shujun and Aganezov Sergey and Wang Xingang and Lippman Zachary B and Schatz Michael C and Soyk Sebastian

Advancing crop genomics requires efficient genetic systems enabled by high-quality personalized genome assemblies. Here, we introduce RagTag, a toolset for automating assembly scaffolding and patching, and we establish chromosome-scale reference genomes for the widely used tomato genotype M82 along with Sweet-100, a new rapid-cycling genotype that we developed to accelerate functional genomics and genome editing in tomato. This work outlines strategies to rapidly expand genetic systems and genomic resources in other plant species.

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Published
December, 2022

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