Diagenode

>>>   Click for Diagenode’s approach to COVID-19

The methylation pattern of DNA and complex correlations with gene expressions during TuMV infection in Chinese cabbage


J. YU , L.-W. GAO , Y. YANG , C. LIU , R.-J. ZHANG , F.-F. SUN , L.-X. SONG , D. XIAO , T.-K. LIU , X.-L. HOU , and C.-W. ZHANG

Chinese cabbage (Brassica rapa L. ssp. pekinensis) is one of the most important economic crops. However, its yield and quality can be severely threatened by Turnip mosaic virus (TuMV). Emerging evidence indicates that epigenetic mechanisms, especially DNA methylation, play an important role in regulating gene expression. Therefore, identification of resistance genes modified by DNA methylation during the virus infection would provide a critical clue for improving disease resistance breeding programs. Here, we present detailed analysis for the correlation of DNA methylation and gene expression involved in several anti-pathogen pathways. We also found that different methylation patterns exist in different methylation sites (CG, CHG, and CHH, where H represents A, G, or T) and genomic regions. Furthermore, we identified disease-resistant genes related to the nucleotide binding site-leucine-rich repeats family, auxin, salicylic acid signaling transduction, cell wall biosynthesis, and protein degradation among the different methylated genes (DMGs) suggesting that these genes may be modified by DNA methylation and work together to activate an immune response. The identified DMGs are a valuable resource for discovering resistance genes. Our study not only provides valuable data for future biotechnology research and epigenetic studies, but also helps to explore how the epigenetic mechanisms modify antiviral pathways.

Tags
MagMeDIP kit

Share this article

Published
May, 2019

Source

Products used in this publication

  • MagMeDIP qPCR Kit box
    C02010021
    MagMeDIP qPCR Kit

Events

  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
  • Dutch Chromatin Meeting
    Radboud UMC, Geert Grooteplein 15, 6525 EZ Nijmegen, The Netherlands, Experience center, route 292 to 307
    Nov 12, 2021
 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics