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BRACHYURY directs histone acetylation to target loci during mesoderm development.


Beisaw A. et al.

T-box transcription factors play essential roles in multiple aspects of vertebrate development. Here, we show that cooperative function of BRACHYURY (T) with histone-modifying enzymes is essential for mouse embryogenesis. A single point mutation (TY88A) results in decreased histone 3 lysine 27 acetylation (H3K27ac) at T target sites, including the T locus, suggesting that T autoregulates the maintenance of its expression and functions by recruiting permissive chromatin modifications to putative enhancers during mesoderm specification. Our data indicate that T mediates H3K27ac recruitment through a physical interaction with p300. In addition, we determine that T plays a prominent role in the specification of hematopoietic and endothelial cell types. Hematopoietic and endothelial gene expression programs are disrupted in TY88A mutant embryos, leading to a defect in the differentiation of hematopoietic progenitors. We show that this role of T is mediated, at least in part, through activation of a distal Lmo2 enhancer.

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Antibody

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Published
January, 2018

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  • ChIP kit icon
    C01010051
    iDeal ChIP-seq kit for Histones
  • Antibody ChIP-seq grade icon
    C15410195
    H3K27me3 Antibody - ChIP-seq Grade
  • Antibody ChIP-seq grade icon
    C15410194
    H3K4me1 Antibody - ChIP-seq Grade

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