Diagenode

Coordinated Transcriptional Control of Adipocyte Triglyceride Lipase (Atgl) by transcription factors Sp1 and PPARγ during Adipocyte Differentiation


Debasish Roy, Kenneth T. Farabaugh, Jing Wu, Alyssa Charrier, Cynthia Smas, Maria Hatzoglou*, Kavitha Thirumurugan and David A. Buchner

The breakdown of stored fat deposits into its components is a highly regulated process that maintains plasma levels of free fatty acids to supply energy to cells. Insulin-mediated transcription of Atgl, the enzyme that mediates the rate-limiting step in lipolysis, is a key point of this regulation. In conditions such as obesity or insulin resistance, Atgl transcription is often misregulated, which can contribute to overall disease progression. The mechanisms by which Atgl is induced during adipogenesis are not fully understood. We utilized computational approaches to identify putative transcriptional regulatory elements in Atgl and then tested the effect of these elements and the transcription factors that bind to them in cultured pre- and mature adipocytes. Herein, we report that Atgl is downregulated by the basal transcription factor Sp1 in preadipocytes, and that the magnitude of downregulation dependents on interactions between Sp1 and PPARγ. In mature adipocytes, when PPARγ is abundant, PPARγ abrogated the transcriptional repression by Sp1 at the Atgl promoter and upregulated Atgl mRNA expression. Targeting the PPARγ-Sp1 interaction could be a potential therapeutic strategy to restore insulin sensitivity by modulating Atgl levels in adipocytes.

Share this article

Published
July, 2017

Source

Related product

  • Bioruptor-pico-next-gen-sequencing
    B01060001
    Bioruptor® Pico sonication device

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics