A lncRNA regulates alternative splicing via establishment of a splicing-specific chromatin signature.

Gonzalez I, Munita R, Agirre E, Dittmer TA, Gysling K, Misteli T, Luco RF

Alternative pre-mRNA splicing is a highly cell type-specific process essential to generating protein diversity. However, the mechanisms responsible for the establishment and maintenance of heritable cell-specific alternative-splicing programs are poorly understood. Recent observations point to a role of histone modifications in the regulation of alternative splicing. Here we report a new mechanism of chromatin-mediated splicing control involving a long noncoding RNA (lncRNA). We have identified an evolutionarily conserved nuclear antisense lncRNA, generated from within the human FGFR2 locus, that promotes epithelial-specific alternative splicing of FGFR2. The lncRNA acts through recruitment of Polycomb-group proteins and the histone demethylase KDM2a to create a chromatin environment that impairs binding of a repressive chromatin-splicing adaptor complex important for mesenchymal-specific splicing. Our results uncover a new function for lncRNAs in the establishment and maintenance of cell-specific alternative splicing via modulation of chromatin signatures.

Chromatin Shearing
H3K27me3 (C15410195)

Share this article

May, 2015


Products used in this publication

  • cut and tag antibody icon
    H3K27me3 Antibody
  • Antibody ChIP icon
    H3K36me2 Antibody


  • EACR 2023
    Torino, Italy
    Jun 12-Jun 15, 2023
 See all events


 See all news

The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics