Diagenode

ChIP-exonuclease in mammalian cells (Prot 66)


Sérandour AA, Carroll JS

ChIP - exonuclease (ChIP - exo) is a high resolution method of genome - wide mapping of DNA - associated proteins that outperforms ChIP - seq by all parameters . We adap ted this method created by Fran k Pugh and Ho Sung Rhee from SOLiD to the Illumina sequencing platform ( Rhee and Pugh, 2011 ; Sérandour et al, 2014) ( cf figure 1) . We provide here a ChIP - exo protocol, optimized on 60 million cells (4 dishes of 15 cm diameter at confluence). Lower cell number has not been tested

Tags
Bioruptor
Chromatin Shearing
Bioruptor Plus

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