Diagenode

ChIP-exonuclease in mammalian cells (Prot 66)


Sérandour AA, Carroll JS

ChIP - exonuclease (ChIP - exo) is a high resolution method of genome - wide mapping of DNA - associated proteins that outperforms ChIP - seq by all parameters . We adap ted this method created by Fran k Pugh and Ho Sung Rhee from SOLiD to the Illumina sequencing platform ( Rhee and Pugh, 2011 ; Sérandour et al, 2014) ( cf figure 1) . We provide here a ChIP - exo protocol, optimized on 60 million cells (4 dishes of 15 cm diameter at confluence). Lower cell number has not been tested

Tags
Bioruptor
Chromatin Shearing
Bioruptor Plus

Share this article

Source

Events

  • CNET 2022
    Gif-Sur-Yvette, France
    Jul 4-Jul 6, 2022
 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics