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<p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p>
<p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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'name' => 'RBBP5 Antibody - ChIP-seq Grade (sample size)',
'description' => 'RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the
MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic
transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation.
It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.',
'clonality' => '',
'isotype' => '',
'lot' => 'A300-109A2',
'concentration' => '1 μg/μl',
'reactivity' => 'Human: positive. Other species: not tested.',
'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IP</td>
<td>10 μg per IP</td>
<td>Fig 4</td>
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<tr>
<td>IHC</td>
<td>1:1,000</td>
<td>Fig 5</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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'name' => 'RBBP5 Antibody',
'description' => '<p><strong>Other names:</strong> RBBP-5, RBQ3, SWD1</p>
<p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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'name' => 'RBBP5 Antibody - ChIP-seq Grade (sample size)',
'description' => 'RBBP5 (UniProtKB/Swiss-Prot entry Q15291) belongs to the highly conserved family of WD-repeat proteins. It’s part of the
MLL1/MLL complex and is as such involved in mono-, di- and trimethylation at lysine 4 of histone H3, a specific tag for epigenetic
transcriptional activation. RBBP5 binds to the retinoblastoma protein thereby playing a role in the regulation of cell proliferation.
It also plays a crucial role in the differentiation potential in embryonic stem (ES) cells.',
'clonality' => '',
'isotype' => '',
'lot' => 'A300-109A2',
'concentration' => '1 μg/μl',
'reactivity' => 'Human: positive. Other species: not tested.',
'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IP</td>
<td>10 μg per IP</td>
<td>Fig 4</td>
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<tr>
<td>IHC</td>
<td>1:1,000</td>
<td>Fig 5</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chip.jpg" alt="RBBP5 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-a.jpg" alt="RBBP5 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-b.jpg" alt="RBBP5 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-c.jpg" alt="RBBP5 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-chipseq-d.jpg" alt="RBBP5 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
</div>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-wb.jpg" alt="RBBP5 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-ip.jpg" alt="RBBP5 Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p>Polyclonal antibody raised in rabbit against human <strong>RBBP5 (RB Binding Protein 5)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against RBBP5 (Cat. No. C15410342) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ACTB and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against RBBP5</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 300 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF2S3 positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against RBBP5</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against RBBP5 (Cat. No. C15410342) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against RBBP5</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 10 μg of the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the RBBP5 antibody diluted 1:500. Lane 3 shows the input (15% of the IP).</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410342-if.jpg" alt="RBBP5 Antibody validated in Immunohistochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunohistochemistry using the Diagenode antibody directed against RBBP5</strong><br />FFPE sections of human colon carcinoma tissue were analysed by Immunohistochemistry with the Diagenode antibody against RBBP5 (Cat. No. C15410342, lane 1) diluted 1:1,000.</small></p>
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