Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b
Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200244). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.