Diagenode

PspCas13b monoclonal antibody (sample size)

Catalog Number
Format
Price
C15200250-10
(C15200244-10)
10 μg
$122.00
  Bulk order
Other format



Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13b) using a recombinant protein.

Lot001
Concentration1 µg/µl
Species reactivityPrevotella sp.
TypeMonoclonal
PurityProtein G purified monoclonal antibody.
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferTBS containing 0.02 % Na-azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western blotting 1:1,000 – 1:4,000 Fig 1, 2
  • Target description

    CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.

  • Validation data

    WB result

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b
    Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.

    WB result

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b
    Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.

  •  Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
  •  Safety sheets
    PspCas13b monoclonal antibody SDS GB en Download
    PspCas13b monoclonal antibody SDS US en Download
    PspCas13b monoclonal antibody SDS DE de Download
    PspCas13b monoclonal antibody SDS JP ja Download
    PspCas13b monoclonal antibody SDS BE nl Download
    PspCas13b monoclonal antibody SDS BE fr Download
    PspCas13b monoclonal antibody SDS FR fr Download
    PspCas13b monoclonal antibody SDS ES es Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PspCas13b monoclonal antibody (sample size) (Diagenode Cat# C15200250-10 Lot# 001). Click here to copy to clipboard.

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