N6-methyladenosine (m6A) polyclonal antibody (sample size)

Catalog Number
10 μg
  Bulk order
Other format

Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.

Concentration1.2 µg/µl
Species reactivityHuman, mouse, other (wide range)
PurityProtein G purified polyclonal antibody.
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide and 0.05% ProClin 300.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
RIP* 2 µg per IP Fig 1
Dot Blotting 1:400 Fig 2

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.

  • Validation data


    Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A
    RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Dot blot

    Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A
    To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.

  •  Applications
    Dot blotting Read more
  •  Documents
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Datasheet m6A C15410208 DATASHEET
    Datasheet description
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: N6-methyladenosine (m6A) polyclonal antibody (sample size) (Diagenode Cat# C15410208-10 Lot# A2125-0010). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Genomewide analysis of 6-methyladenine DNA in peripheral blood mononuclear cells of systemic lupus erythematosus.
    Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y
    AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral bloo...


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