Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A
RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).