Diagenode

>>>   Click for Diagenode’s approach to COVID-19

m66A Antibody (sample size)

Catalog Number
Format
Price
C15410365-10
10 µg
$95.00
  Bulk order
Other format



Full name: N6N6-dimethyladenosine polyclonal antibody

Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (m66A) conjugated to BSA.

LotA3001-0010
Concentration2.4 µg/µl
Species reactivityHuman, mouse, other (wide range): positive
TypePolyclonal
PurityProtein A purified polyclonal antibody
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
  • Validation data
    A.
    m66A Antibody validated for RIP

    B.
    m66A Antibody validated in RIP

    Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m66A
    RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using 2 µg of the Diagenode antibody against m66A (cat. No. C15410365) or with an equal amount of mouse IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1A shows the Bioanalyzer profile obtained with the m66A antibody (left) The right panel shows the input. Figure 1B shows the gel image for the m66A antibody, the IgG negative control and the input (lane 1, 2 and 3, respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

    m66A Antibody validated for RIP

    Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m66A
    RIP assays were performed on 40 µg total RNA from human HeLa cells using the Diagenode m66A antibody (cat. nr. C15410365). A titration of the antibody consisting of 1, 2 and 5 µg per RIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QRT-PCR was performed with primers for the 18s and 28s rRNA genes. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    m66A Antibody Dot Blot Validation

    Figure 3. Dot blot analysis using the Diagenode antibody directed against m66A
    To demonstrate the specificity of the Diagenode antibody against m66A (cat. No. C15410365), a Dot Blot analysis was performed using in vitro transcribed RNA molecules containing m6A or m66A modified bases as well as an unmodified control RNA. 5 and 2.5 ng of the respective RNA molecules were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6N6-dimethyladenosine.

  • Target Description

    N6N6-dimethyladenosine (m66A) is a modified base which is mainly present in 18s rRNA.

  •  Documents
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    m66A polyclonal antibody - Datasheet DATASHEET
    Polyclonal antibody raised in rabbit against N6N6-dimethyladenosine (m66A) conjugated to BSA. 
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: m66A Antibody (sample size) (Diagenode Cat# C15410365-10 Lot# A3001-0010). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics