Diagenode

HA tag monoclonal antibody - Classic

Antibody ChIP icon
Catalog Number
Format
Price
C15200190
(MAb-190-050)
50 μg/25 μl
$305.00
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Monoclonal antibody raised in mouse against the HA tag (amino acid sequence YPYDVPDYA) using a KLH-conjugated synthetic peptide. The HA antibody recognizes the influenza Hemagglutinin epitope which is extensively used as a protein tag in expression vectors. This antibody is extremely specific and allows unambiguous identification and quantitative analysis of HA tagged proteins.

Lot003
Concentration1.0 µg/µl
Species reactivitySpecific for HA-tagged fusion proteins expressed in bacteria, insect, mammalian cells
TypeMonoclonal
PurityProtein A purified
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
Western Blotting 1:1,000 - 1:3,000 Fig 1
IP/ChIP 1:150 - 1:200
IS 1:500 - 1:1,000

* Please note that optimal antibody amount should be determined by the end-user for each specific assay condition.

  • Validation Data

    Western Blot

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the HA protein tag
    Western blot was performed on whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the Diagenode monoclonal antibody directed against HA (Cat. No. MAb-190-050). The antibody was used at a dilution of 1:1,000. Figure 1 shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet HAtag MAb-190-050 DATASHEET
    Datasheet description
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: HA tag monoclonal antibody - Classic (Diagenode Cat# C15200190 Lot# 003). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    FBXO32 promotes microenvironment underlying epithelial-mesenchymal transition via CtBP1 during tumour metastasis and brain development
    Sahu S.K. et al.
    The set of events that convert adherent epithelial cells into migratory cells are collectively known as epithelial-mesenchymal transition (EMT). EMT is involved during development, for example, in triggering neural crest migration, and in pathogenesis such as metastasis. Here we discover FBXO32, an E3 ubiquitin liga...

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