Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'en',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'product' => array(
'Product' => array(
'id' => '2811',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody (sample size)',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9).</p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200231-10',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '120',
'price_USD' => '125',
'price_GBP' => '115',
'price_JPY' => '17700',
'price_CNY' => '0',
'price_AUD' => '315',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231-10-ug',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:34',
'created' => '2016-05-20 14:37:55',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => '477',
'name' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '2.2 μg/μl',
'reactivity' => 'Streptococcus pyogenes',
'type' => 'Monoclonal',
'purity' => 'Protein G purified',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:6,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:100</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:41:10',
'created' => '2016-05-20 14:21:02',
'select_label' => '477 - CRISPR/Cas9 C-terminal monoclonal antibody (001 - 2.2 μg/μl - Streptococcus pyogenes - Protein G purified - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/en/p/crispr-cas9-c-terminal-monoclonal-antibody-100-ugg'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.'
$meta_title = 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode'
$product = array(
'Product' => array(
'id' => '2811',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody (sample size)',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9).</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200231-10',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '120',
'price_USD' => '125',
'price_GBP' => '115',
'price_JPY' => '17700',
'price_CNY' => '0',
'price_AUD' => '315',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231-10-ug',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:34',
'created' => '2016-05-20 14:37:55',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => '477',
'name' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '2.2 μg/μl',
'reactivity' => 'Streptococcus pyogenes',
'type' => 'Monoclonal',
'purity' => 'Protein G purified',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:6,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:100</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:41:10',
'created' => '2016-05-20 14:21:02',
'select_label' => '477 - CRISPR/Cas9 C-terminal monoclonal antibody (001 - 2.2 μg/μl - Streptococcus pyogenes - Protein G purified - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '181',
'name' => 'CRISPR/Cas9 C-terminal C15200231',
'product_id' => '2960',
'modified' => '2018-03-01 14:43:30',
'created' => '2016-05-20 14:39:11'
),
'Master' => array(
'id' => '2960',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of<strong> Cas9</strong> <strong>nuclease (CRISPR-associated protein 9)</strong>.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '100 μg',
'catalog_number' => 'C15200231-100',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '525',
'price_USD' => '550',
'price_GBP' => '475',
'price_JPY' => '76400',
'price_CNY' => '0',
'price_AUD' => '1375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-100-ugg',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, WB and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:22',
'created' => '2018-03-01 14:41:57'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '122',
'position' => '1',
'parent_id' => '100',
'name' => '<em>S. pyogenes</EM> CRISPR/Cas9 antibodies',
'description' => '<p>Diagenode offers the broad range of antibodies raised against the N- or C-terminus of the Cas9 nuclease from <em>Streptococcus <g class="gr_ gr_5 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="5" data-gr-id="5">pyogenes</g></em>. These highly specific polyclonal and monoclonal antibodies are validated in Western blot, immunoprecipitation, immunofluorescence and in chromatin immunoprecipitation.</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2><em><a name="pyogenes"></a>S. pyogenes</em> CRISPR/Cas9 antibodies<a></a></h2>
<div class="panel">
<h2>Discover our first monoclonal CRISPR/Cas9 antibody validated in ChIP<br /><br /></h2>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="/img/landing-pages/crispr-cas9-chip-on-hih3t3.jpg" alt="" /></div>
<div class="small-7 medium-7 large-7 columns">
<ul>
<li>Validated in chromatin immunoprecipitation</li>
<li>Performs better than FLAG antibody</li>
<li>Excellent for WB, IF and IP</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 µg chromatin was incubated overnight at 4°C with 5 or 10 µg of either an anti-FLAG antibody or the Diagenode antibody against Cas9 (Cat. No. C15200229). Mouse IgG was used as a negative IP control. qPCR was performed with primers specific for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns text-right"><a href="/p/crispr-cas9-monoclonal-antibody-50-ug-25-μl" class="tiny details button radius">Learn more</a></div>
</div>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>First ChIP-grade CRISPR/Cas9 polyclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/c_a_s9-chip-grade-antibody.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Excellent polyclonal antibody for chromatin immunoprecipitation</li>
<li>Optimized for highest ChIP specificity and yields</li>
<li>Validated for all applications including immunoblotting, immunofluorescence and western blot</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP- H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated with either 5 μg of an anti-FLAG antibody or 2 μl of the Diagenode antibody against Cas9. The pre-immune serum (PPI) was used as negative IP control. Then qPCR was performed with primers specific for the GFP gene, and for two non-targeted regions: Ppap2c and Prkcd, used as negative controls. This figure shows the recovery, expressed as a % of input.</small></p>
<p class="text-right"><a href="../p/crispr-cas9-polyclonal-antibody" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 monoclonal antibody 4G10</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/cas9_4g10_fig1.png" width="170" height="302" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against N-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong>Immunofluorescence</strong>: Hela cells were transiently transfected with a Cas9 expression vector. The cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA. The cells were stained with the Cas9 antibody at 4°C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT. Nuclei were counter-stained with Hoechst 33342 (right).</small></p>
<p class="text-right"><a href="../p/crispr-cas9-monoclonal-antibody-4g10-50-ug" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 C-terminal monoclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against C-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong> Western blot</strong> was performed on 20 μg protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9. The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
<p class="text-right"><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug" class="details tiny button">Learn more</a></p>
</div>
<div class="small-12 medium-12 large-12 columns">
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<a name="table"></a>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IF</th>
<th>IP</th>
<th>ChIP</th>
<th>Antibody raised against</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="../p/crispr-cas9-monoclonal-antibody-50-ug-25-μl"><strong class="diacol">CRISPR/Cas9 monoclonal antibody</strong></a></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><span class="diacol">N-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-polyclonal-antibody">CRISPR/Cas9 polyclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td><strong class="diacol">+++</strong></td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-4g10-50-ug">CRISPR/Cas9 monoclonal antibody 4G10</a></td>
<td>+++</td>
<td>+++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul"><strong class="diacol">CRISPR/Cas9 C-terminal monoclonal antibody</strong></a> <span class="label alert" style="font-size: 0.9rem;">NEW!</span></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">no</strong></td>
<td><strong class="diacol">+</strong></td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug">CRISPR/Cas9 C-terminal monoclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>+</td>
<td>no</td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-7A9-50-mg">CRISPR/Cas9 monoclonal antibody 7A9</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-hrp-monoclonal-antibody-50-ul">CRISPR/Cas9 - HRP monoclonal antibody 7A9</a></td>
<td>+++</td>
<td>no</td>
<td>no</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
</tbody>
</table>
</div>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 's-pyogenes-crispr-cas9-antibodies',
'cookies_tag_id' => '1',
'meta_keywords' => '',
'meta_description' => 'S.pyogenes CRISPR/Cas9 antibodies',
'meta_title' => 'S.pyogenes CRISPR/Cas9 antibodies',
'modified' => '2018-03-29 11:58:22',
'created' => '2016-09-09 10:39:20',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2021-07-01 10:22:38',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '901',
'name' => 'Datasheet CRISPR/Cas9 C-terminal monoclonal antibody C15200231',
'description' => '<p>Datasheet of the CRISPR/Cas9 C-terminal monoclonal antibody <span>raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9). </span></p>
<div class="page" title="Page 1">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/Datasheet_C15200231_crispr-cas9-c-terminal.pdf',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-datasheet',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-05-25 14:50:06',
'created' => '2016-05-20 15:12:31',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4399',
'name' => 'A self-inactivating system for AAV-mediated in vivo base editing',
'authors' => 'Zuo Y. et al.',
'description' => '<p>DNA base editors have been harnessed as an exciting therapeutic platform for human diseases and are rapidly progressing into human clinical trials. However, persistent expression of base editors delivered via adeno-associated virus (AAV) poses concerns with specificity and immunogenicity. Here we develop selfinactivating base editor (siBE) systems with a negative feedback loop where one guide RNA (gRNA) targets the gene of interest and the other targets the deaminase domain itself. We demonstrate that siBE confers efficient on-target editing with time-dependent self-inactivation and increased editing specificity. For the in vivo utilization, we further employ the intein split approach to package siBE targeting mouse Angptl3 into AAV9. Systemic delivery of AAV9-siBE confer efficient editing of Angptl3 in liver, resulting in reduced serum levels of ANGPTL3, triglyceride and total cholesterol, with the active base editor undetectable at 8 weeks after administration. These self-inactivating base editing systems are highly promising for future therapeutic applications.</p>',
'date' => '0000-00-00',
'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1',
'doi' => '10.21203/rs.3.rs-1663604/v1',
'modified' => '2022-08-11 15:24:23',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '494',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2020-07-01 11:41:56',
'created' => '2020-07-01 11:41:56',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '496',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2020-07-01 11:43:12',
'created' => '2020-07-01 11:43:12',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '490',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 11:39:49',
'created' => '2020-07-01 11:38:30',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '489',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 11:37:13',
'created' => '2020-07-01 11:37:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '493',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2020-07-01 11:41:24',
'created' => '2020-07-01 11:41:24',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '492',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 11:40:48',
'created' => '2020-07-01 11:40:48',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '491',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2020-07-01 11:39:28',
'created' => '2020-07-01 11:39:28',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '495',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 11:42:33',
'created' => '2020-07-01 11:42:33',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/en/p/crispr-cas9-c-terminal-monoclonal-antibody-100-ugg'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2960',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of<strong> Cas9</strong> <strong>nuclease (CRISPR-associated protein 9)</strong>.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '100 μg',
'catalog_number' => 'C15200231-100',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '525',
'price_USD' => '550',
'price_GBP' => '475',
'price_JPY' => '76400',
'price_CNY' => '0',
'price_AUD' => '1375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-100-ugg',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, WB and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:22',
'created' => '2018-03-01 14:41:57'
),
(int) 1 => array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
)
$pro = array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
'id' => '4412',
'product_id' => '2811',
'application_id' => '29'
)
)
$slugs = array(
(int) 0 => 'immunofluorescence'
)
$applications = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'locale' => 'eng'
)
$description = '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>'
$name = 'IF'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
'id' => '2093',
'product_id' => '2811',
'document_id' => '38'
)
)
$sds = array(
'id' => '495',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 11:42:33',
'created' => '2020-07-01 11:42:33',
'ProductsSafetySheet' => array(
'id' => '940',
'product_id' => '2811',
'safety_sheet_id' => '495'
)
)
$publication = array(
'id' => '4399',
'name' => 'A self-inactivating system for AAV-mediated in vivo base editing',
'authors' => 'Zuo Y. et al.',
'description' => '<p>DNA base editors have been harnessed as an exciting therapeutic platform for human diseases and are rapidly progressing into human clinical trials. However, persistent expression of base editors delivered via adeno-associated virus (AAV) poses concerns with specificity and immunogenicity. Here we develop selfinactivating base editor (siBE) systems with a negative feedback loop where one guide RNA (gRNA) targets the gene of interest and the other targets the deaminase domain itself. We demonstrate that siBE confers efficient on-target editing with time-dependent self-inactivation and increased editing specificity. For the in vivo utilization, we further employ the intein split approach to package siBE targeting mouse Angptl3 into AAV9. Systemic delivery of AAV9-siBE confer efficient editing of Angptl3 in liver, resulting in reduced serum levels of ANGPTL3, triglyceride and total cholesterol, with the active base editor undetectable at 8 weeks after administration. These self-inactivating base editing systems are highly promising for future therapeutic applications.</p>',
'date' => '0000-00-00',
'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1',
'doi' => '10.21203/rs.3.rs-1663604/v1',
'modified' => '2022-08-11 15:24:23',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
'id' => '6090',
'product_id' => '2811',
'publication_id' => '4399'
)
)
$externalLink = ' <a href="https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'en',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'product' => array(
'Product' => array(
'id' => '2811',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody (sample size)',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9).</p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200231-10',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '120',
'price_USD' => '125',
'price_GBP' => '115',
'price_JPY' => '17700',
'price_CNY' => '0',
'price_AUD' => '315',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231-10-ug',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:34',
'created' => '2016-05-20 14:37:55',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => '477',
'name' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '2.2 μg/μl',
'reactivity' => 'Streptococcus pyogenes',
'type' => 'Monoclonal',
'purity' => 'Protein G purified',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:6,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:100</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:41:10',
'created' => '2016-05-20 14:21:02',
'select_label' => '477 - CRISPR/Cas9 C-terminal monoclonal antibody (001 - 2.2 μg/μl - Streptococcus pyogenes - Protein G purified - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/en/p/crispr-cas9-c-terminal-monoclonal-antibody-100-ugg'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.'
$meta_title = 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode'
$product = array(
'Product' => array(
'id' => '2811',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody (sample size)',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9).</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200231-10',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '120',
'price_USD' => '125',
'price_GBP' => '115',
'price_JPY' => '17700',
'price_CNY' => '0',
'price_AUD' => '315',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231-10-ug',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:34',
'created' => '2016-05-20 14:37:55',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => '477',
'name' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '2.2 μg/μl',
'reactivity' => 'Streptococcus pyogenes',
'type' => 'Monoclonal',
'purity' => 'Protein G purified',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:6,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:100</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:41:10',
'created' => '2016-05-20 14:21:02',
'select_label' => '477 - CRISPR/Cas9 C-terminal monoclonal antibody (001 - 2.2 μg/μl - Streptococcus pyogenes - Protein G purified - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '181',
'name' => 'CRISPR/Cas9 C-terminal C15200231',
'product_id' => '2960',
'modified' => '2018-03-01 14:43:30',
'created' => '2016-05-20 14:39:11'
),
'Master' => array(
'id' => '2960',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of<strong> Cas9</strong> <strong>nuclease (CRISPR-associated protein 9)</strong>.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '100 μg',
'catalog_number' => 'C15200231-100',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '525',
'price_USD' => '550',
'price_GBP' => '475',
'price_JPY' => '76400',
'price_CNY' => '0',
'price_AUD' => '1375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-100-ugg',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, WB and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:22',
'created' => '2018-03-01 14:41:57'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '122',
'position' => '1',
'parent_id' => '100',
'name' => '<em>S. pyogenes</EM> CRISPR/Cas9 antibodies',
'description' => '<p>Diagenode offers the broad range of antibodies raised against the N- or C-terminus of the Cas9 nuclease from <em>Streptococcus <g class="gr_ gr_5 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="5" data-gr-id="5">pyogenes</g></em>. These highly specific polyclonal and monoclonal antibodies are validated in Western blot, immunoprecipitation, immunofluorescence and in chromatin immunoprecipitation.</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2><em><a name="pyogenes"></a>S. pyogenes</em> CRISPR/Cas9 antibodies<a></a></h2>
<div class="panel">
<h2>Discover our first monoclonal CRISPR/Cas9 antibody validated in ChIP<br /><br /></h2>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="/img/landing-pages/crispr-cas9-chip-on-hih3t3.jpg" alt="" /></div>
<div class="small-7 medium-7 large-7 columns">
<ul>
<li>Validated in chromatin immunoprecipitation</li>
<li>Performs better than FLAG antibody</li>
<li>Excellent for WB, IF and IP</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 µg chromatin was incubated overnight at 4°C with 5 or 10 µg of either an anti-FLAG antibody or the Diagenode antibody against Cas9 (Cat. No. C15200229). Mouse IgG was used as a negative IP control. qPCR was performed with primers specific for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns text-right"><a href="/p/crispr-cas9-monoclonal-antibody-50-ug-25-μl" class="tiny details button radius">Learn more</a></div>
</div>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>First ChIP-grade CRISPR/Cas9 polyclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/c_a_s9-chip-grade-antibody.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Excellent polyclonal antibody for chromatin immunoprecipitation</li>
<li>Optimized for highest ChIP specificity and yields</li>
<li>Validated for all applications including immunoblotting, immunofluorescence and western blot</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP- H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated with either 5 μg of an anti-FLAG antibody or 2 μl of the Diagenode antibody against Cas9. The pre-immune serum (PPI) was used as negative IP control. Then qPCR was performed with primers specific for the GFP gene, and for two non-targeted regions: Ppap2c and Prkcd, used as negative controls. This figure shows the recovery, expressed as a % of input.</small></p>
<p class="text-right"><a href="../p/crispr-cas9-polyclonal-antibody" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 monoclonal antibody 4G10</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/cas9_4g10_fig1.png" width="170" height="302" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against N-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong>Immunofluorescence</strong>: Hela cells were transiently transfected with a Cas9 expression vector. The cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA. The cells were stained with the Cas9 antibody at 4°C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT. Nuclei were counter-stained with Hoechst 33342 (right).</small></p>
<p class="text-right"><a href="../p/crispr-cas9-monoclonal-antibody-4g10-50-ug" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 C-terminal monoclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against C-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong> Western blot</strong> was performed on 20 μg protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9. The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
<p class="text-right"><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug" class="details tiny button">Learn more</a></p>
</div>
<div class="small-12 medium-12 large-12 columns">
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<a name="table"></a>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IF</th>
<th>IP</th>
<th>ChIP</th>
<th>Antibody raised against</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="../p/crispr-cas9-monoclonal-antibody-50-ug-25-μl"><strong class="diacol">CRISPR/Cas9 monoclonal antibody</strong></a></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><span class="diacol">N-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-polyclonal-antibody">CRISPR/Cas9 polyclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td><strong class="diacol">+++</strong></td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-4g10-50-ug">CRISPR/Cas9 monoclonal antibody 4G10</a></td>
<td>+++</td>
<td>+++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul"><strong class="diacol">CRISPR/Cas9 C-terminal monoclonal antibody</strong></a> <span class="label alert" style="font-size: 0.9rem;">NEW!</span></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">no</strong></td>
<td><strong class="diacol">+</strong></td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug">CRISPR/Cas9 C-terminal monoclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>+</td>
<td>no</td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-7A9-50-mg">CRISPR/Cas9 monoclonal antibody 7A9</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-hrp-monoclonal-antibody-50-ul">CRISPR/Cas9 - HRP monoclonal antibody 7A9</a></td>
<td>+++</td>
<td>no</td>
<td>no</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
</tbody>
</table>
</div>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 's-pyogenes-crispr-cas9-antibodies',
'cookies_tag_id' => '1',
'meta_keywords' => '',
'meta_description' => 'S.pyogenes CRISPR/Cas9 antibodies',
'meta_title' => 'S.pyogenes CRISPR/Cas9 antibodies',
'modified' => '2018-03-29 11:58:22',
'created' => '2016-09-09 10:39:20',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2021-07-01 10:22:38',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '901',
'name' => 'Datasheet CRISPR/Cas9 C-terminal monoclonal antibody C15200231',
'description' => '<p>Datasheet of the CRISPR/Cas9 C-terminal monoclonal antibody <span>raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9). </span></p>
<div class="page" title="Page 1">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/Datasheet_C15200231_crispr-cas9-c-terminal.pdf',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-datasheet',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-05-25 14:50:06',
'created' => '2016-05-20 15:12:31',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4399',
'name' => 'A self-inactivating system for AAV-mediated in vivo base editing',
'authors' => 'Zuo Y. et al.',
'description' => '<p>DNA base editors have been harnessed as an exciting therapeutic platform for human diseases and are rapidly progressing into human clinical trials. However, persistent expression of base editors delivered via adeno-associated virus (AAV) poses concerns with specificity and immunogenicity. Here we develop selfinactivating base editor (siBE) systems with a negative feedback loop where one guide RNA (gRNA) targets the gene of interest and the other targets the deaminase domain itself. We demonstrate that siBE confers efficient on-target editing with time-dependent self-inactivation and increased editing specificity. For the in vivo utilization, we further employ the intein split approach to package siBE targeting mouse Angptl3 into AAV9. Systemic delivery of AAV9-siBE confer efficient editing of Angptl3 in liver, resulting in reduced serum levels of ANGPTL3, triglyceride and total cholesterol, with the active base editor undetectable at 8 weeks after administration. These self-inactivating base editing systems are highly promising for future therapeutic applications.</p>',
'date' => '0000-00-00',
'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1',
'doi' => '10.21203/rs.3.rs-1663604/v1',
'modified' => '2022-08-11 15:24:23',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '494',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2020-07-01 11:41:56',
'created' => '2020-07-01 11:41:56',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '496',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2020-07-01 11:43:12',
'created' => '2020-07-01 11:43:12',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '490',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 11:39:49',
'created' => '2020-07-01 11:38:30',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '489',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 11:37:13',
'created' => '2020-07-01 11:37:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '493',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2020-07-01 11:41:24',
'created' => '2020-07-01 11:41:24',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '492',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 11:40:48',
'created' => '2020-07-01 11:40:48',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '491',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2020-07-01 11:39:28',
'created' => '2020-07-01 11:39:28',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '495',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 11:42:33',
'created' => '2020-07-01 11:42:33',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/en/p/crispr-cas9-c-terminal-monoclonal-antibody-100-ugg'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2960',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of<strong> Cas9</strong> <strong>nuclease (CRISPR-associated protein 9)</strong>.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '100 μg',
'catalog_number' => 'C15200231-100',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '525',
'price_USD' => '550',
'price_GBP' => '475',
'price_JPY' => '76400',
'price_CNY' => '0',
'price_AUD' => '1375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-100-ugg',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, WB and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:22',
'created' => '2018-03-01 14:41:57'
),
(int) 1 => array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
)
$pro = array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
'id' => '4412',
'product_id' => '2811',
'application_id' => '29'
)
)
$slugs = array(
(int) 0 => 'immunofluorescence'
)
$applications = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'locale' => 'eng'
)
$description = '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>'
$name = 'IF'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
'id' => '2093',
'product_id' => '2811',
'document_id' => '38'
)
)
$sds = array(
'id' => '495',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 11:42:33',
'created' => '2020-07-01 11:42:33',
'ProductsSafetySheet' => array(
'id' => '940',
'product_id' => '2811',
'safety_sheet_id' => '495'
)
)
$publication = array(
'id' => '4399',
'name' => 'A self-inactivating system for AAV-mediated in vivo base editing',
'authors' => 'Zuo Y. et al.',
'description' => '<p>DNA base editors have been harnessed as an exciting therapeutic platform for human diseases and are rapidly progressing into human clinical trials. However, persistent expression of base editors delivered via adeno-associated virus (AAV) poses concerns with specificity and immunogenicity. Here we develop selfinactivating base editor (siBE) systems with a negative feedback loop where one guide RNA (gRNA) targets the gene of interest and the other targets the deaminase domain itself. We demonstrate that siBE confers efficient on-target editing with time-dependent self-inactivation and increased editing specificity. For the in vivo utilization, we further employ the intein split approach to package siBE targeting mouse Angptl3 into AAV9. Systemic delivery of AAV9-siBE confer efficient editing of Angptl3 in liver, resulting in reduced serum levels of ANGPTL3, triglyceride and total cholesterol, with the active base editor undetectable at 8 weeks after administration. These self-inactivating base editing systems are highly promising for future therapeutic applications.</p>',
'date' => '0000-00-00',
'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1',
'doi' => '10.21203/rs.3.rs-1663604/v1',
'modified' => '2022-08-11 15:24:23',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
'id' => '6090',
'product_id' => '2811',
'publication_id' => '4399'
)
)
$externalLink = ' <a href="https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'en',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'product' => array(
'Product' => array(
'id' => '2811',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody (sample size)',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9).</p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200231-10',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '120',
'price_USD' => '125',
'price_GBP' => '115',
'price_JPY' => '17700',
'price_CNY' => '0',
'price_AUD' => '315',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231-10-ug',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:34',
'created' => '2016-05-20 14:37:55',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => '477',
'name' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '2.2 μg/μl',
'reactivity' => 'Streptococcus pyogenes',
'type' => 'Monoclonal',
'purity' => 'Protein G purified',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:6,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:100</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:41:10',
'created' => '2016-05-20 14:21:02',
'select_label' => '477 - CRISPR/Cas9 C-terminal monoclonal antibody (001 - 2.2 μg/μl - Streptococcus pyogenes - Protein G purified - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/en/p/crispr-cas9-c-terminal-monoclonal-antibody-100-ugg'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.'
$meta_title = 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode'
$product = array(
'Product' => array(
'id' => '2811',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody (sample size)',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9).</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200231-10',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '120',
'price_USD' => '125',
'price_GBP' => '115',
'price_JPY' => '17700',
'price_CNY' => '0',
'price_AUD' => '315',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231-10-ug',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:34',
'created' => '2016-05-20 14:37:55',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => '477',
'name' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '2.2 μg/μl',
'reactivity' => 'Streptococcus pyogenes',
'type' => 'Monoclonal',
'purity' => 'Protein G purified',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:6,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:100</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:41:10',
'created' => '2016-05-20 14:21:02',
'select_label' => '477 - CRISPR/Cas9 C-terminal monoclonal antibody (001 - 2.2 μg/μl - Streptococcus pyogenes - Protein G purified - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '181',
'name' => 'CRISPR/Cas9 C-terminal C15200231',
'product_id' => '2960',
'modified' => '2018-03-01 14:43:30',
'created' => '2016-05-20 14:39:11'
),
'Master' => array(
'id' => '2960',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of<strong> Cas9</strong> <strong>nuclease (CRISPR-associated protein 9)</strong>.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '100 μg',
'catalog_number' => 'C15200231-100',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '525',
'price_USD' => '550',
'price_GBP' => '475',
'price_JPY' => '76400',
'price_CNY' => '0',
'price_AUD' => '1375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-100-ugg',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, WB and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:22',
'created' => '2018-03-01 14:41:57'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '122',
'position' => '1',
'parent_id' => '100',
'name' => '<em>S. pyogenes</EM> CRISPR/Cas9 antibodies',
'description' => '<p>Diagenode offers the broad range of antibodies raised against the N- or C-terminus of the Cas9 nuclease from <em>Streptococcus <g class="gr_ gr_5 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="5" data-gr-id="5">pyogenes</g></em>. These highly specific polyclonal and monoclonal antibodies are validated in Western blot, immunoprecipitation, immunofluorescence and in chromatin immunoprecipitation.</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2><em><a name="pyogenes"></a>S. pyogenes</em> CRISPR/Cas9 antibodies<a></a></h2>
<div class="panel">
<h2>Discover our first monoclonal CRISPR/Cas9 antibody validated in ChIP<br /><br /></h2>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="/img/landing-pages/crispr-cas9-chip-on-hih3t3.jpg" alt="" /></div>
<div class="small-7 medium-7 large-7 columns">
<ul>
<li>Validated in chromatin immunoprecipitation</li>
<li>Performs better than FLAG antibody</li>
<li>Excellent for WB, IF and IP</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 µg chromatin was incubated overnight at 4°C with 5 or 10 µg of either an anti-FLAG antibody or the Diagenode antibody against Cas9 (Cat. No. C15200229). Mouse IgG was used as a negative IP control. qPCR was performed with primers specific for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns text-right"><a href="/p/crispr-cas9-monoclonal-antibody-50-ug-25-μl" class="tiny details button radius">Learn more</a></div>
</div>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>First ChIP-grade CRISPR/Cas9 polyclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/c_a_s9-chip-grade-antibody.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Excellent polyclonal antibody for chromatin immunoprecipitation</li>
<li>Optimized for highest ChIP specificity and yields</li>
<li>Validated for all applications including immunoblotting, immunofluorescence and western blot</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP- H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated with either 5 μg of an anti-FLAG antibody or 2 μl of the Diagenode antibody against Cas9. The pre-immune serum (PPI) was used as negative IP control. Then qPCR was performed with primers specific for the GFP gene, and for two non-targeted regions: Ppap2c and Prkcd, used as negative controls. This figure shows the recovery, expressed as a % of input.</small></p>
<p class="text-right"><a href="../p/crispr-cas9-polyclonal-antibody" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 monoclonal antibody 4G10</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/cas9_4g10_fig1.png" width="170" height="302" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against N-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong>Immunofluorescence</strong>: Hela cells were transiently transfected with a Cas9 expression vector. The cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA. The cells were stained with the Cas9 antibody at 4°C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT. Nuclei were counter-stained with Hoechst 33342 (right).</small></p>
<p class="text-right"><a href="../p/crispr-cas9-monoclonal-antibody-4g10-50-ug" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 C-terminal monoclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against C-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong> Western blot</strong> was performed on 20 μg protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9. The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
<p class="text-right"><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug" class="details tiny button">Learn more</a></p>
</div>
<div class="small-12 medium-12 large-12 columns">
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<a name="table"></a>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IF</th>
<th>IP</th>
<th>ChIP</th>
<th>Antibody raised against</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="../p/crispr-cas9-monoclonal-antibody-50-ug-25-μl"><strong class="diacol">CRISPR/Cas9 monoclonal antibody</strong></a></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><span class="diacol">N-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-polyclonal-antibody">CRISPR/Cas9 polyclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td><strong class="diacol">+++</strong></td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-4g10-50-ug">CRISPR/Cas9 monoclonal antibody 4G10</a></td>
<td>+++</td>
<td>+++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul"><strong class="diacol">CRISPR/Cas9 C-terminal monoclonal antibody</strong></a> <span class="label alert" style="font-size: 0.9rem;">NEW!</span></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">no</strong></td>
<td><strong class="diacol">+</strong></td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug">CRISPR/Cas9 C-terminal monoclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>+</td>
<td>no</td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-7A9-50-mg">CRISPR/Cas9 monoclonal antibody 7A9</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-hrp-monoclonal-antibody-50-ul">CRISPR/Cas9 - HRP monoclonal antibody 7A9</a></td>
<td>+++</td>
<td>no</td>
<td>no</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
</tbody>
</table>
</div>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 's-pyogenes-crispr-cas9-antibodies',
'cookies_tag_id' => '1',
'meta_keywords' => '',
'meta_description' => 'S.pyogenes CRISPR/Cas9 antibodies',
'meta_title' => 'S.pyogenes CRISPR/Cas9 antibodies',
'modified' => '2018-03-29 11:58:22',
'created' => '2016-09-09 10:39:20',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2021-07-01 10:22:38',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '901',
'name' => 'Datasheet CRISPR/Cas9 C-terminal monoclonal antibody C15200231',
'description' => '<p>Datasheet of the CRISPR/Cas9 C-terminal monoclonal antibody <span>raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9). </span></p>
<div class="page" title="Page 1">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/Datasheet_C15200231_crispr-cas9-c-terminal.pdf',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-datasheet',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-05-25 14:50:06',
'created' => '2016-05-20 15:12:31',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4399',
'name' => 'A self-inactivating system for AAV-mediated in vivo base editing',
'authors' => 'Zuo Y. et al.',
'description' => '<p>DNA base editors have been harnessed as an exciting therapeutic platform for human diseases and are rapidly progressing into human clinical trials. However, persistent expression of base editors delivered via adeno-associated virus (AAV) poses concerns with specificity and immunogenicity. Here we develop selfinactivating base editor (siBE) systems with a negative feedback loop where one guide RNA (gRNA) targets the gene of interest and the other targets the deaminase domain itself. We demonstrate that siBE confers efficient on-target editing with time-dependent self-inactivation and increased editing specificity. For the in vivo utilization, we further employ the intein split approach to package siBE targeting mouse Angptl3 into AAV9. Systemic delivery of AAV9-siBE confer efficient editing of Angptl3 in liver, resulting in reduced serum levels of ANGPTL3, triglyceride and total cholesterol, with the active base editor undetectable at 8 weeks after administration. These self-inactivating base editing systems are highly promising for future therapeutic applications.</p>',
'date' => '0000-00-00',
'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1',
'doi' => '10.21203/rs.3.rs-1663604/v1',
'modified' => '2022-08-11 15:24:23',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '494',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2020-07-01 11:41:56',
'created' => '2020-07-01 11:41:56',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '496',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2020-07-01 11:43:12',
'created' => '2020-07-01 11:43:12',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '490',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 11:39:49',
'created' => '2020-07-01 11:38:30',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '489',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 11:37:13',
'created' => '2020-07-01 11:37:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '493',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2020-07-01 11:41:24',
'created' => '2020-07-01 11:41:24',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '492',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 11:40:48',
'created' => '2020-07-01 11:40:48',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '491',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2020-07-01 11:39:28',
'created' => '2020-07-01 11:39:28',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '495',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 11:42:33',
'created' => '2020-07-01 11:42:33',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/en/p/crispr-cas9-c-terminal-monoclonal-antibody-100-ugg'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2960',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of<strong> Cas9</strong> <strong>nuclease (CRISPR-associated protein 9)</strong>.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '100 μg',
'catalog_number' => 'C15200231-100',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '525',
'price_USD' => '550',
'price_GBP' => '475',
'price_JPY' => '76400',
'price_CNY' => '0',
'price_AUD' => '1375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-100-ugg',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, WB and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:22',
'created' => '2018-03-01 14:41:57'
),
(int) 1 => array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
)
$pro = array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
'id' => '4412',
'product_id' => '2811',
'application_id' => '29'
)
)
$slugs = array(
(int) 0 => 'immunofluorescence'
)
$applications = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'locale' => 'eng'
)
$description = '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>'
$name = 'IF'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
'id' => '2093',
'product_id' => '2811',
'document_id' => '38'
)
)
$sds = array(
'id' => '495',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 11:42:33',
'created' => '2020-07-01 11:42:33',
'ProductsSafetySheet' => array(
'id' => '940',
'product_id' => '2811',
'safety_sheet_id' => '495'
)
)
$publication = array(
'id' => '4399',
'name' => 'A self-inactivating system for AAV-mediated in vivo base editing',
'authors' => 'Zuo Y. et al.',
'description' => '<p>DNA base editors have been harnessed as an exciting therapeutic platform for human diseases and are rapidly progressing into human clinical trials. However, persistent expression of base editors delivered via adeno-associated virus (AAV) poses concerns with specificity and immunogenicity. Here we develop selfinactivating base editor (siBE) systems with a negative feedback loop where one guide RNA (gRNA) targets the gene of interest and the other targets the deaminase domain itself. We demonstrate that siBE confers efficient on-target editing with time-dependent self-inactivation and increased editing specificity. For the in vivo utilization, we further employ the intein split approach to package siBE targeting mouse Angptl3 into AAV9. Systemic delivery of AAV9-siBE confer efficient editing of Angptl3 in liver, resulting in reduced serum levels of ANGPTL3, triglyceride and total cholesterol, with the active base editor undetectable at 8 weeks after administration. These self-inactivating base editing systems are highly promising for future therapeutic applications.</p>',
'date' => '0000-00-00',
'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1',
'doi' => '10.21203/rs.3.rs-1663604/v1',
'modified' => '2022-08-11 15:24:23',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
'id' => '6090',
'product_id' => '2811',
'publication_id' => '4399'
)
)
$externalLink = ' <a href="https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'en',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'product' => array(
'Product' => array(
'id' => '2811',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody (sample size)',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9).</p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200231-10',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '120',
'price_USD' => '125',
'price_GBP' => '115',
'price_JPY' => '17700',
'price_CNY' => '0',
'price_AUD' => '315',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231-10-ug',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:34',
'created' => '2016-05-20 14:37:55',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => '477',
'name' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '2.2 μg/μl',
'reactivity' => 'Streptococcus pyogenes',
'type' => 'Monoclonal',
'purity' => 'Protein G purified',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:6,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:100</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:41:10',
'created' => '2016-05-20 14:21:02',
'select_label' => '477 - CRISPR/Cas9 C-terminal monoclonal antibody (001 - 2.2 μg/μl - Streptococcus pyogenes - Protein G purified - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/en/p/crispr-cas9-c-terminal-monoclonal-antibody-100-ugg'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.'
$meta_title = 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode'
$product = array(
'Product' => array(
'id' => '2811',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody (sample size)',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9).</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200231-10',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '120',
'price_USD' => '125',
'price_GBP' => '115',
'price_JPY' => '17700',
'price_CNY' => '0',
'price_AUD' => '315',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231-10-ug',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR , IF and WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:34',
'created' => '2016-05-20 14:37:55',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => '477',
'name' => 'CRISPR/Cas9 C-terminal monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '2.2 μg/μl',
'reactivity' => 'Streptococcus pyogenes',
'type' => 'Monoclonal',
'purity' => 'Protein G purified',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:6,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:100</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-C15200231',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-08-03 16:41:10',
'created' => '2016-05-20 14:21:02',
'select_label' => '477 - CRISPR/Cas9 C-terminal monoclonal antibody (001 - 2.2 μg/μl - Streptococcus pyogenes - Protein G purified - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '181',
'name' => 'CRISPR/Cas9 C-terminal C15200231',
'product_id' => '2960',
'modified' => '2018-03-01 14:43:30',
'created' => '2016-05-20 14:39:11'
),
'Master' => array(
'id' => '2960',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of<strong> Cas9</strong> <strong>nuclease (CRISPR-associated protein 9)</strong>.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '100 μg',
'catalog_number' => 'C15200231-100',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '525',
'price_USD' => '550',
'price_GBP' => '475',
'price_JPY' => '76400',
'price_CNY' => '0',
'price_AUD' => '1375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-100-ugg',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, WB and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:22',
'created' => '2018-03-01 14:41:57'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '122',
'position' => '1',
'parent_id' => '100',
'name' => '<em>S. pyogenes</EM> CRISPR/Cas9 antibodies',
'description' => '<p>Diagenode offers the broad range of antibodies raised against the N- or C-terminus of the Cas9 nuclease from <em>Streptococcus <g class="gr_ gr_5 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="5" data-gr-id="5">pyogenes</g></em>. These highly specific polyclonal and monoclonal antibodies are validated in Western blot, immunoprecipitation, immunofluorescence and in chromatin immunoprecipitation.</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h2><em><a name="pyogenes"></a>S. pyogenes</em> CRISPR/Cas9 antibodies<a></a></h2>
<div class="panel">
<h2>Discover our first monoclonal CRISPR/Cas9 antibody validated in ChIP<br /><br /></h2>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="/img/landing-pages/crispr-cas9-chip-on-hih3t3.jpg" alt="" /></div>
<div class="small-7 medium-7 large-7 columns">
<ul>
<li>Validated in chromatin immunoprecipitation</li>
<li>Performs better than FLAG antibody</li>
<li>Excellent for WB, IF and IP</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 µg chromatin was incubated overnight at 4°C with 5 or 10 µg of either an anti-FLAG antibody or the Diagenode antibody against Cas9 (Cat. No. C15200229). Mouse IgG was used as a negative IP control. qPCR was performed with primers specific for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns text-right"><a href="/p/crispr-cas9-monoclonal-antibody-50-ug-25-μl" class="tiny details button radius">Learn more</a></div>
</div>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>First ChIP-grade CRISPR/Cas9 polyclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/c_a_s9-chip-grade-antibody.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Excellent polyclonal antibody for chromatin immunoprecipitation</li>
<li>Optimized for highest ChIP specificity and yields</li>
<li>Validated for all applications including immunoblotting, immunofluorescence and western blot</li>
</ul>
<p><small><strong>ChIP</strong> was performed on NIH3T3 cells stably expressing GFP- H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated with either 5 μg of an anti-FLAG antibody or 2 μl of the Diagenode antibody against Cas9. The pre-immune serum (PPI) was used as negative IP control. Then qPCR was performed with primers specific for the GFP gene, and for two non-targeted regions: Ppap2c and Prkcd, used as negative controls. This figure shows the recovery, expressed as a % of input.</small></p>
<p class="text-right"><a href="../p/crispr-cas9-polyclonal-antibody" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 monoclonal antibody 4G10</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/landing-pages/cas9_4g10_fig1.png" width="170" height="302" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against N-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong>Immunofluorescence</strong>: Hela cells were transiently transfected with a Cas9 expression vector. The cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA. The cells were stained with the Cas9 antibody at 4°C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT. Nuclei were counter-stained with Hoechst 33342 (right).</small></p>
<p class="text-right"><a href="../p/crispr-cas9-monoclonal-antibody-4g10-50-ug" class="details tiny button">Learn more</a></p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>CRISPR/Cas9 C-terminal monoclonal antibody</h3>
</div>
<div class="small-4 medium-4 large-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200223-IP.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<ul>
<li>Antibody raised against C-terminus of Cas9 nuclease</li>
<li>Validated for western blot, IP and immunofluorescence</li>
</ul>
<p><small><strong> Western blot</strong> was performed on 20 μg protein extracts from Cas9 expressing HeLa cells (lane 1) and on negative control HeLa cells (lane 2) with the Diagenode antibody against Cas9. The antibody was diluted 1:4,000. The marker is shown on the left, position of the Cas9 protein is indicated on the right. </small></p>
<p class="text-right"><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug" class="details tiny button">Learn more</a></p>
</div>
<div class="small-12 medium-12 large-12 columns">
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<a name="table"></a>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IF</th>
<th>IP</th>
<th>ChIP</th>
<th>Antibody raised against</th>
</tr>
</thead>
<tbody>
<tr>
<td><a href="../p/crispr-cas9-monoclonal-antibody-50-ug-25-μl"><strong class="diacol">CRISPR/Cas9 monoclonal antibody</strong></a></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><span class="diacol">N-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-polyclonal-antibody">CRISPR/Cas9 polyclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td><strong class="diacol">+++</strong></td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-4g10-50-ug">CRISPR/Cas9 monoclonal antibody 4G10</a></td>
<td>+++</td>
<td>+++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="../p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul"><strong class="diacol">CRISPR/Cas9 C-terminal monoclonal antibody</strong></a> <span class="label alert" style="font-size: 0.9rem;">NEW!</span></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">+++</strong></td>
<td><strong class="diacol">no</strong></td>
<td><strong class="diacol">+</strong></td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-c-terminal-monoclonal-antibody-50-ug">CRISPR/Cas9 C-terminal monoclonal antibody</a></td>
<td>++</td>
<td>++</td>
<td>+</td>
<td>no</td>
<td><span class="diacol">C-terminus of Cas9 nuclease</span></td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-monoclonal-antibody-7A9-50-mg">CRISPR/Cas9 monoclonal antibody 7A9</a></td>
<td>++</td>
<td>++</td>
<td>++</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
<tr>
<td><a href="/p/crispr-cas9-hrp-monoclonal-antibody-50-ul">CRISPR/Cas9 - HRP monoclonal antibody 7A9</a></td>
<td>+++</td>
<td>no</td>
<td>no</td>
<td>no</td>
<td>N-terminus of Cas9 nuclease</td>
</tr>
</tbody>
</table>
</div>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 's-pyogenes-crispr-cas9-antibodies',
'cookies_tag_id' => '1',
'meta_keywords' => '',
'meta_description' => 'S.pyogenes CRISPR/Cas9 antibodies',
'meta_title' => 'S.pyogenes CRISPR/Cas9 antibodies',
'modified' => '2018-03-29 11:58:22',
'created' => '2016-09-09 10:39:20',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '127',
'position' => '10',
'parent_id' => '4',
'name' => 'ChIP-grade antibodies',
'description' => '<div class="row">
<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'chip-grade-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
'modified' => '2021-07-01 10:22:38',
'created' => '2017-02-14 11:16:04',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '901',
'name' => 'Datasheet CRISPR/Cas9 C-terminal monoclonal antibody C15200231',
'description' => '<p>Datasheet of the CRISPR/Cas9 C-terminal monoclonal antibody <span>raised in mouse against the C-terminus of Cas9 nuclease (CRISPR-associated protein 9). </span></p>
<div class="page" title="Page 1">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/Datasheet_C15200231_crispr-cas9-c-terminal.pdf',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-datasheet',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-05-25 14:50:06',
'created' => '2016-05-20 15:12:31',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4399',
'name' => 'A self-inactivating system for AAV-mediated in vivo base editing',
'authors' => 'Zuo Y. et al.',
'description' => '<p>DNA base editors have been harnessed as an exciting therapeutic platform for human diseases and are rapidly progressing into human clinical trials. However, persistent expression of base editors delivered via adeno-associated virus (AAV) poses concerns with specificity and immunogenicity. Here we develop selfinactivating base editor (siBE) systems with a negative feedback loop where one guide RNA (gRNA) targets the gene of interest and the other targets the deaminase domain itself. We demonstrate that siBE confers efficient on-target editing with time-dependent self-inactivation and increased editing specificity. For the in vivo utilization, we further employ the intein split approach to package siBE targeting mouse Angptl3 into AAV9. Systemic delivery of AAV9-siBE confer efficient editing of Angptl3 in liver, resulting in reduced serum levels of ANGPTL3, triglyceride and total cholesterol, with the active base editor undetectable at 8 weeks after administration. These self-inactivating base editing systems are highly promising for future therapeutic applications.</p>',
'date' => '0000-00-00',
'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1',
'doi' => '10.21203/rs.3.rs-1663604/v1',
'modified' => '2022-08-11 15:24:23',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '494',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2020-07-01 11:41:56',
'created' => '2020-07-01 11:41:56',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '496',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2020-07-01 11:43:12',
'created' => '2020-07-01 11:43:12',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '490',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 11:39:49',
'created' => '2020-07-01 11:38:30',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '489',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 11:37:13',
'created' => '2020-07-01 11:37:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '493',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2020-07-01 11:41:24',
'created' => '2020-07-01 11:41:24',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '492',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 11:40:48',
'created' => '2020-07-01 11:40:48',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '491',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2020-07-01 11:39:28',
'created' => '2020-07-01 11:39:28',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '495',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 11:42:33',
'created' => '2020-07-01 11:42:33',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/en/p/crispr-cas9-c-terminal-monoclonal-antibody-100-ugg'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2960',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against the C-terminus of<strong> Cas9</strong> <strong>nuclease (CRISPR-associated protein 9)</strong>.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200231-chip-fig1.png" alt="CRISPR/Cas9 C-terminal Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP using the Diagenode monoclonal antibody directed against Cas9</strong><br />ChIP was performed on NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA. 50 μg chromatin was incubated overnight at 4°C with 5 μg of either the Diagenode antibody against Cas9 (cat. No. C15200231) or the anti-blue antibody (C15200213), used as a negative IP control. qPCR was performed with primers speci c for the GFP gene, and for a non-targeted region (protein kinase C delta, Prkcd), used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-wb.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Western Blot" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong><br />Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 2) or on untransfected control cells (lane 1) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200231). The antibody was diluted 1:6,000 in TBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15210231-if.jpg" alt="CRISPR/Cas9 C-terminal Antibody validated in Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong> <br />HeLa cells expressing Cas9 under the control of the tight TRE promoter and untransfected control cells were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200231) diluted 1:100, followed by incubation with a donkey anti-mouse secondary antibody coupled to AF488 (upper panel). Nuclei were counter-stained with Hoechst 33342 (lower panel).</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. Recently, the CRISPR/Cas9 (CRISPR-associated protein 9 nuclease, UniProtKB/Swiss-Prot entry Q99ZW2) system from S. pyogenes has been adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.</p>',
'label3' => '',
'info3' => '',
'format' => '100 μg',
'catalog_number' => 'C15200231-100',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '525',
'price_USD' => '550',
'price_GBP' => '475',
'price_JPY' => '76400',
'price_CNY' => '0',
'price_AUD' => '1375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-100-ugg',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, WB and IF. Batch-specific data available on the website. Sample size available.',
'modified' => '2021-12-23 12:19:22',
'created' => '2018-03-01 14:41:57'
),
(int) 1 => array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
)
$pro = array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2810',
'antibody_id' => '477',
'name' => 'CRISPR/Cas9 C-terminal Antibody',
'description' => '',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 μg/23 μl',
'catalog_number' => 'C15200231-50',
'old_catalog_number' => '',
'sf_code' => 'C15200231-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '425',
'price_USD' => '445',
'price_GBP' => '400',
'price_JPY' => '61600',
'price_CNY' => '0',
'price_AUD' => '1115',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'crispr-cas9-c-terminal-monoclonal-antibody-50-ug-23-ul',
'meta_title' => 'CRISPR/Cas9 C-terminal Antibody - ChIP Grade (C15200231) | Diagenode',
'meta_keywords' => ' CRISPR/Cas9 C-terminal monoclonal antibody, ChIP, Western blot, IF, Diagenode',
'meta_description' => 'S. pyogenes CRISPR/Cas9 C-terminal Monoclonal Antibody validated in ChIP-qPCR, IF and WB. Batch-specific data available on the website. Sample size available',
'modified' => '2021-12-23 12:19:45',
'created' => '2016-05-20 14:26:43',
'ProductsGroup' => array(
'id' => '277',
'product_id' => '2810',
'group_id' => '181'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'ProductsApplication' => array(
'id' => '4412',
'product_id' => '2811',
'application_id' => '29'
)
)
$slugs = array(
(int) 0 => 'immunofluorescence'
)
$applications = array(
'id' => '29',
'position' => '10',
'parent_id' => '40',
'name' => 'IF',
'description' => '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunofluorescence',
'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications',
'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-27 16:23:10',
'created' => '2015-07-08 13:46:02',
'locale' => 'eng'
)
$description = '<p><strong>Immunofluorescence</strong>:</p>
<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>'
$name = 'IF'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
'id' => '2093',
'product_id' => '2811',
'document_id' => '38'
)
)
$sds = array(
'id' => '495',
'name' => 'CRISPR/Cas9 C-terminal antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/CRISPR-Cas9/SDS-C15200231-CRISPR_Cas9_C-terminal_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 11:42:33',
'created' => '2020-07-01 11:42:33',
'ProductsSafetySheet' => array(
'id' => '940',
'product_id' => '2811',
'safety_sheet_id' => '495'
)
)
$publication = array(
'id' => '4399',
'name' => 'A self-inactivating system for AAV-mediated in vivo base editing',
'authors' => 'Zuo Y. et al.',
'description' => '<p>DNA base editors have been harnessed as an exciting therapeutic platform for human diseases and are rapidly progressing into human clinical trials. However, persistent expression of base editors delivered via adeno-associated virus (AAV) poses concerns with specificity and immunogenicity. Here we develop selfinactivating base editor (siBE) systems with a negative feedback loop where one guide RNA (gRNA) targets the gene of interest and the other targets the deaminase domain itself. We demonstrate that siBE confers efficient on-target editing with time-dependent self-inactivation and increased editing specificity. For the in vivo utilization, we further employ the intein split approach to package siBE targeting mouse Angptl3 into AAV9. Systemic delivery of AAV9-siBE confer efficient editing of Angptl3 in liver, resulting in reduced serum levels of ANGPTL3, triglyceride and total cholesterol, with the active base editor undetectable at 8 weeks after administration. These self-inactivating base editing systems are highly promising for future therapeutic applications.</p>',
'date' => '0000-00-00',
'pmid' => 'https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1',
'doi' => '10.21203/rs.3.rs-1663604/v1',
'modified' => '2022-08-11 15:24:23',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
'id' => '6090',
'product_id' => '2811',
'publication_id' => '4399'
)
)
$externalLink = ' <a href="https://doi.org/10.21203%2Frs.3.rs-1663604%2Fv1" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
