Diagenode

CRBN (Cereblon) monoclonal antibody

Catalog Number
Format
Price
C15200245
50 µg
$315.00
  Bulk order
Other format

Monoclonal antibody raised in mouse against the N-terminus of the CRBN (Cereblon). Diagenode Cereblon antibody, in contrast to most antibodies on the market, possesses excellent imunoprecipitating properties (see validation data).

Lot001
Concentration1 µg/µl
Species reactivityHuman: positive. Other species: not tested.
TypeMonoclonal
PurityProtein G purified monoclonal antibody.
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferTBS containing 0.02 % Na-azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western blotting 1:1,000 Fig 1
IP 1:200 Fig 2
  • Validation data

    WB result

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN
    Western blot was performed on protein extracts from wild type MOLT4 cells (lane 2) or MOLT4-CRBN-/- cells (lane 1) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:300 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.

    WB result

    Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN
    IP was performed on whole cell extracts (400 µg) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 µg) is shown lane 1 and 2.

  •  Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  •  Documents
    CRBN monoclonal antibody datasheet DATASHEET
    Monoclonal antibody raised in mouse against the N-terminus of the CRBN (Cereblon).
    Download
  •  Publications

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