The most "popular couple" of Histone modifications: H3K27me3 and H3K4me3...
Looking into chromatin can be a complicated story. Transcriptional control is dependent on a huge network of epigenetic modifications.
For the last 9 years, it's been Diagenode and academic partners full time job to establish standards in this field (and still is).
So, if you're looking to start your epigenetic research and generate a number of high impact data, you'll want to consider the most validated reagents, antibodies, DNA or Chromatin shearing devices or automated workstations for performing consistent ChIP assays.
ChIP/ChIP-seq grade antibodies, the critical key to Chromatin Immoprecipitation success
When asked about how they choose their ChIP antibody of interest, researchers often point to their prior experiences with the antibody, comparative results, and well-cited products. However, researchers may not necessarily be satisfied with antibody performance. Oftentimes, they must optimize each and every ChIP assay with a suboptimal antibody simply to maintain "consistency" in future experiments even if better antibodies become available.
Diagenode has manufactured, quality-verified, and validated hundreds of polyclonal or monoclonal antisera and selected a fraction of them as actual high quality standards. Over time Diagenode became an expert aid for the 'production and validation' in many active epigenetic consortiums such as the recent EU project "Blueprint". Production of ChIP-Seq grade reagents and methods is one of our main challenges today.