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Sequence of the methylated spike-in control in FASTA format – Premium RRBS kit v2

This document and files are to process the sequencing data from the spike-in controls included in the RRBS Kit v2 in order to estimate the bisulfite conversion efficiency. You can find the sequences of the controls and the methylation status of each cytosine in the Premium RRBS Kit v2 Manual.

However, to ease the data processing, we provide three files that can be downloaded from the Premium RRBS Kit v2 page :

  • RRBS_methylated_control.fa: the sequence of the methylated spike-in control in FASTA format
  • RRBS_unmethylated_control.fa: the sequence of the unmethylated spike-in control in FASTA format
  • RRBS_control_unmC.bed: the positions of the unmethylated cytosines in the sequence of the methylated control in BED format

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