MicroPlex Library Preparation Kit v2 Single Index Plate (SIP) contains 48 unique Indexing Reagents pre-dispensed and sealed in a microplate. The Indexing Reagents consist of amplification primers containing Illumina®-compatible single indexes, each with a unique 8nt Sanger index sequence developed by the Wellcome Trust Sanger Institute in Cambridge, UK (see next page). Each well of the SIP contains sufficient volume of the Indexing Reagent for a single use. No more than 4 freeze/thaw cycles are recommended for the SIP. The plate is sealed with foil that can be pierced with a multichannel pipet tip to collect the required amount of index to assemble the reactions.