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Lot | A2125-0010 |
---|---|
Concentration | 1.2 µg/µl |
Species reactivity | Human, mouse, other (wide range): positive. |
Type | Polyclonal |
Purity | Protein G purified polyclonal antibody. |
Host | Rabbit |
Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
RIP/RIP-seq* | 1-2 µg per IP | Fig 1, 2, 3 |
Dot Blotting | 1:400 | Fig 4 |
*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.
Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A
RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A
RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.
Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
A.
B.
C.
D.
Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A
RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).
Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A
To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.
Datasheet m6A C15410208 DATASHEET Datasheet description | Download |
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
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SDS C15410208 N6 methyladenosine m6A Antibody JP ja | Download |
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How to properly cite this product in your workDiagenode strongly recommends using this: N6-methyladenosine (m6A) antibody - RIP-seq Grade (Diagenode Cat# C15410208-50 Lot# A2125-0010). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Genomewide analysis of 6-methyladenine DNA in peripheral blood mononuclear cells of systemic lupus erythematosus. |
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RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. 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RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. 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Batch-specific data available on the website. Sample size available.' $meta_title = 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2286', 'antibody_id' => '315', 'name' => 'N6-methyladenosine (m6A) antibody - RIP-seq Grade', 'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-01-17 17:03:33', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '315', 'name' => 'm6A polyclonal antibody', 'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.', 'clonality' => '', 'isotype' => '', 'lot' => 'A2125-0010', 'concentration' => '1.2 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive.', 'type' => 'Polyclonal', 'purity' => 'Protein G purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP/RIP-seq*</td> <td>1-2 µg per IP</td> <td>Fig 1, 2, 3</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide and 0.05% ProClin 300.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-02-26 10:52:35', 'created' => '0000-00-00 00:00:00', 'select_label' => '315 - m6A polyclonal antibody (A2125-0010 - 1.2 µg/µl - Human, mouse, other (wide range): positive. - Protein G purified polyclonal antibody. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '216', 'name' => 'C15410208', 'product_id' => '2286', 'modified' => '2017-06-22 12:04:50', 'created' => '2017-06-22 12:04:50' ) ), 'Group' => array( 'Group' => array( 'id' => '216', 'name' => 'C15410208', 'product_id' => '2286', 'modified' => '2017-06-22 12:04:50', 'created' => '2017-06-22 12:04:50' ), 'Master' => array( 'id' => '2286', 'antibody_id' => '315', 'name' => 'N6-methyladenosine (m6A) Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-01-17 17:03:33', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '109', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA modifications', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). 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METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1856', 'product_id' => '2286', 'document_id' => '38' ) ) $sds = array( 'id' => '3633', 'name' => 'SDS C15410208 N6 methyladenosine m6A Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/m6A/SDS-C15410208-N6-methyladenosine_m6A_Antibody-BE-nl-GHS_3_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 17:02:45', 'created' => '2024-01-17 17:02:45', 'ProductsSafetySheet' => array( 'id' => '5933', 'product_id' => '2286', 'safety_sheet_id' => '3633' ) ) $publication = array( 'id' => '3689', 'name' => 'Genomewide analysis of 6-methyladenine DNA in peripheral blood mononuclear cells of systemic lupus erythematosus.', 'authors' => 'Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y', 'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>', 'date' => '2019-03-01', 'pmid' => 'http://www.pubmed.gov/30744524', 'doi' => '10.1177/0961203319828520', 'modified' => '2019-06-28 13:53:56', 'created' => '2019-06-21 14:55:31', 'ProductsPublication' => array( 'id' => '3743', 'product_id' => '2286', 'publication_id' => '3689' ) ) $externalLink = ' <a href="http://www.pubmed.gov/30744524" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2286', 'antibody_id' => '315', 'name' => 'N6-methyladenosine (m6A) antibody - RIP-seq Grade', 'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. 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Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.', 'clonality' => '', 'isotype' => '', 'lot' => 'A2125-0010', 'concentration' => '1.2 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive.', 'type' => 'Polyclonal', 'purity' => 'Protein G purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP/RIP-seq*</td> <td>1-2 µg per IP</td> <td>Fig 1, 2, 3</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide and 0.05% ProClin 300.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-02-26 10:52:35', 'created' => '0000-00-00 00:00:00', 'select_label' => '315 - m6A polyclonal antibody (A2125-0010 - 1.2 µg/µl - Human, mouse, other (wide range): positive. - Protein G purified polyclonal antibody. - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/cn/p/m6a-polyclonal-antibody-pioneer-50-mg' ) $language = 'cn' $meta_keywords = 'N6-methyladenosine (m6A),polyclonal antibody ' $meta_description = 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.' $meta_title = 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2286', 'antibody_id' => '315', 'name' => 'N6-methyladenosine (m6A) antibody - RIP-seq Grade', 'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-01-17 17:03:33', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '315', 'name' => 'm6A polyclonal antibody', 'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.', 'clonality' => '', 'isotype' => '', 'lot' => 'A2125-0010', 'concentration' => '1.2 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive.', 'type' => 'Polyclonal', 'purity' => 'Protein G purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP/RIP-seq*</td> <td>1-2 µg per IP</td> <td>Fig 1, 2, 3</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide and 0.05% ProClin 300.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-02-26 10:52:35', 'created' => '0000-00-00 00:00:00', 'select_label' => '315 - m6A polyclonal antibody (A2125-0010 - 1.2 µg/µl - Human, mouse, other (wide range): positive. - Protein G purified polyclonal antibody. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '216', 'name' => 'C15410208', 'product_id' => '2286', 'modified' => '2017-06-22 12:04:50', 'created' => '2017-06-22 12:04:50' ) ), 'Group' => array( 'Group' => array( 'id' => '216', 'name' => 'C15410208', 'product_id' => '2286', 'modified' => '2017-06-22 12:04:50', 'created' => '2017-06-22 12:04:50' ), 'Master' => array( 'id' => '2286', 'antibody_id' => '315', 'name' => 'N6-methyladenosine (m6A) Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-01-17 17:03:33', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '109', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA modifications', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '376', 'name' => 'Datasheet m6A C15410208', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_m6A_C15410208.pdf', 'slug' => 'datasheet-m6a-c15410208', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3689', 'name' => 'Genomewide analysis of 6-methyladenine DNA in peripheral blood mononuclear cells of systemic lupus erythematosus.', 'authors' => 'Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y', 'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>', 'date' => '2019-03-01', 'pmid' => 'http://www.pubmed.gov/30744524', 'doi' => '10.1177/0961203319828520', 'modified' => '2019-06-28 13:53:56', 'created' => '2019-06-21 14:55:31', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3627', 'name' => 'SDS C15410208 N6 methyladenosine m6A Antibody US en', 'language' => 'en', 'url' => 'files/SDS/m6A/SDS-C15410208-N6-methyladenosine_m6A_Antibody-US-en-GHS_3_0.pdf', 'countries' => 'US', 'modified' => '2024-01-17 16:58:05', 'created' => '2024-01-17 16:57:45', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3629', 'name' => 'SDS C15410208 N6 methyladenosine m6A Antibody GB en', 'language' => 'en', 'url' => 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Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Sample size available.', 'modified' => '2024-01-17 17:03:08', 'created' => '2017-06-22 12:04:15', 'ProductsGroup' => array( 'id' => '241', 'product_id' => '2902', 'group_id' => '216' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( 'id' => '4961', 'product_id' => '2286', 'application_id' => '36' ) ) $slugs = array( (int) 0 => 'rip' ) $applications = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'locale' => 'zho' ) $description = '<p>RIP</p>' $name = 'RIP' $document = array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1856', 'product_id' => '2286', 'document_id' => '38' ) ) $sds = array( 'id' => '3633', 'name' => 'SDS C15410208 N6 methyladenosine m6A Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/m6A/SDS-C15410208-N6-methyladenosine_m6A_Antibody-BE-nl-GHS_3_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 17:02:45', 'created' => '2024-01-17 17:02:45', 'ProductsSafetySheet' => array( 'id' => '5933', 'product_id' => '2286', 'safety_sheet_id' => '3633' ) ) $publication = array( 'id' => '3689', 'name' => 'Genomewide analysis of 6-methyladenine DNA in peripheral blood mononuclear cells of systemic lupus erythematosus.', 'authors' => 'Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y', 'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>', 'date' => '2019-03-01', 'pmid' => 'http://www.pubmed.gov/30744524', 'doi' => '10.1177/0961203319828520', 'modified' => '2019-06-28 13:53:56', 'created' => '2019-06-21 14:55:31', 'ProductsPublication' => array( 'id' => '3743', 'product_id' => '2286', 'publication_id' => '3689' ) ) $externalLink = ' <a href="http://www.pubmed.gov/30744524" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2286', 'antibody_id' => '315', 'name' => 'N6-methyladenosine (m6A) antibody - RIP-seq Grade', 'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. 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Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide and 0.05% ProClin 300.', 'precautions' => 'This product is for research use only. 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Batch-specific data available on the website. Sample size available.' $meta_title = 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2286', 'antibody_id' => '315', 'name' => 'N6-methyladenosine (m6A) antibody - RIP-seq Grade', 'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-01-17 17:03:33', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '315', 'name' => 'm6A polyclonal antibody', 'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.', 'clonality' => '', 'isotype' => '', 'lot' => 'A2125-0010', 'concentration' => '1.2 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive.', 'type' => 'Polyclonal', 'purity' => 'Protein G purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP/RIP-seq*</td> <td>1-2 µg per IP</td> <td>Fig 1, 2, 3</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide and 0.05% ProClin 300.', 'precautions' => 'This product is for research use only. 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RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-01-17 17:03:33', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '109', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA modifications', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). 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METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1856', 'product_id' => '2286', 'document_id' => '38' ) ) $sds = array( 'id' => '3633', 'name' => 'SDS C15410208 N6 methyladenosine m6A Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/m6A/SDS-C15410208-N6-methyladenosine_m6A_Antibody-BE-nl-GHS_3_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 17:02:45', 'created' => '2024-01-17 17:02:45', 'ProductsSafetySheet' => array( 'id' => '5933', 'product_id' => '2286', 'safety_sheet_id' => '3633' ) ) $publication = array( 'id' => '3689', 'name' => 'Genomewide analysis of 6-methyladenine DNA in peripheral blood mononuclear cells of systemic lupus erythematosus.', 'authors' => 'Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y', 'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>', 'date' => '2019-03-01', 'pmid' => 'http://www.pubmed.gov/30744524', 'doi' => '10.1177/0961203319828520', 'modified' => '2019-06-28 13:53:56', 'created' => '2019-06-21 14:55:31', 'ProductsPublication' => array( 'id' => '3743', 'product_id' => '2286', 'publication_id' => '3689' ) ) $externalLink = ' <a href="http://www.pubmed.gov/30744524" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2286', 'antibody_id' => '315', 'name' => 'N6-methyladenosine (m6A) antibody - RIP-seq Grade', 'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. 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Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.', 'clonality' => '', 'isotype' => '', 'lot' => 'A2125-0010', 'concentration' => '1.2 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive.', 'type' => 'Polyclonal', 'purity' => 'Protein G purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP/RIP-seq*</td> <td>1-2 µg per IP</td> <td>Fig 1, 2, 3</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide and 0.05% ProClin 300.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-02-26 10:52:35', 'created' => '0000-00-00 00:00:00', 'select_label' => '315 - m6A polyclonal antibody (A2125-0010 - 1.2 µg/µl - Human, mouse, other (wide range): positive. - Protein G purified polyclonal antibody. - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/cn/p/m6a-polyclonal-antibody-pioneer-50-mg' ) $language = 'cn' $meta_keywords = 'N6-methyladenosine (m6A),polyclonal antibody ' $meta_description = 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.' $meta_title = 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2286', 'antibody_id' => '315', 'name' => 'N6-methyladenosine (m6A) antibody - RIP-seq Grade', 'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-01-17 17:03:33', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '315', 'name' => 'm6A polyclonal antibody', 'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.', 'clonality' => '', 'isotype' => '', 'lot' => 'A2125-0010', 'concentration' => '1.2 µg/µl', 'reactivity' => 'Human, mouse, other (wide range): positive.', 'type' => 'Polyclonal', 'purity' => 'Protein G purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP/RIP-seq*</td> <td>1-2 µg per IP</td> <td>Fig 1, 2, 3</td> </tr> <tr> <td>Dot Blotting</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 0.05% azide and 0.05% ProClin 300.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-02-26 10:52:35', 'created' => '0000-00-00 00:00:00', 'select_label' => '315 - m6A polyclonal antibody (A2125-0010 - 1.2 µg/µl - Human, mouse, other (wide range): positive. - Protein G purified polyclonal antibody. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '216', 'name' => 'C15410208', 'product_id' => '2286', 'modified' => '2017-06-22 12:04:50', 'created' => '2017-06-22 12:04:50' ) ), 'Group' => array( 'Group' => array( 'id' => '216', 'name' => 'C15410208', 'product_id' => '2286', 'modified' => '2017-06-22 12:04:50', 'created' => '2017-06-22 12:04:50' ), 'Master' => array( 'id' => '2286', 'antibody_id' => '315', 'name' => 'N6-methyladenosine (m6A) Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p> <p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p></p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410208-50', 'old_catalog_number' => '', 'sf_code' => 'C15410208-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 11, 2020', 'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg', 'meta_title' => 'N6-methyladenosine (m6A) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ', 'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-01-17 17:03:33', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '109', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA modifications', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'rna-modifications-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody', 'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies', 'meta_title' => 'RNA modifications antibodies | Diagenode.com', 'modified' => '2020-09-01 11:43:28', 'created' => '2016-02-15 10:58:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '376', 'name' => 'Datasheet m6A C15410208', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_m6A_C15410208.pdf', 'slug' => 'datasheet-m6a-c15410208', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3689', 'name' => 'Genomewide analysis of 6-methyladenine DNA in peripheral blood mononuclear cells of systemic lupus erythematosus.', 'authors' => 'Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y', 'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>', 'date' => '2019-03-01', 'pmid' => 'http://www.pubmed.gov/30744524', 'doi' => '10.1177/0961203319828520', 'modified' => '2019-06-28 13:53:56', 'created' => '2019-06-21 14:55:31', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3627', 'name' => 'SDS C15410208 N6 methyladenosine m6A Antibody US en', 'language' => 'en', 'url' => 'files/SDS/m6A/SDS-C15410208-N6-methyladenosine_m6A_Antibody-US-en-GHS_3_0.pdf', 'countries' => 'US', 'modified' => '2024-01-17 16:58:05', 'created' => '2024-01-17 16:57:45', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3629', 'name' => 'SDS C15410208 N6 methyladenosine m6A Antibody GB en', 'language' => 'en', 'url' => 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Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Sample size available.', 'modified' => '2024-01-17 17:03:08', 'created' => '2017-06-22 12:04:15', 'ProductsGroup' => array( 'id' => '241', 'product_id' => '2902', 'group_id' => '216' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'ProductsApplication' => array( 'id' => '4961', 'product_id' => '2286', 'application_id' => '36' ) ) $slugs = array( (int) 0 => 'rip' ) $applications = array( 'id' => '36', 'position' => '10', 'parent_id' => '40', 'name' => 'RIP', 'description' => '<p>RIP</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'rip', 'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode', 'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.', 'meta_title' => 'RIP antibodies | Diagenode', 'modified' => '2016-01-21 08:09:31', 'created' => '2015-07-08 13:54:45', 'locale' => 'zho' ) $description = '<p>RIP</p>' $name = 'RIP' $document = array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1856', 'product_id' => '2286', 'document_id' => '38' ) ) $sds = array( 'id' => '3633', 'name' => 'SDS C15410208 N6 methyladenosine m6A Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/m6A/SDS-C15410208-N6-methyladenosine_m6A_Antibody-BE-nl-GHS_3_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-17 17:02:45', 'created' => '2024-01-17 17:02:45', 'ProductsSafetySheet' => array( 'id' => '5933', 'product_id' => '2286', 'safety_sheet_id' => '3633' ) ) $publication = array( 'id' => '3689', 'name' => 'Genomewide analysis of 6-methyladenine DNA in peripheral blood mononuclear cells of systemic lupus erythematosus.', 'authors' => 'Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y', 'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>', 'date' => '2019-03-01', 'pmid' => 'http://www.pubmed.gov/30744524', 'doi' => '10.1177/0961203319828520', 'modified' => '2019-06-28 13:53:56', 'created' => '2019-06-21 14:55:31', 'ProductsPublication' => array( 'id' => '3743', 'product_id' => '2286', 'publication_id' => '3689' ) ) $externalLink = ' <a href="http://www.pubmed.gov/30744524" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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