H4pan polyclonal antibody

50 μg/39 μl
  Bulk order

Polyclonal antibody raised in rabbit against histone H4 using a KLH-conjugated synthetic peptide containing an unmodified sequence from the central part of the protein.

Concentration1.3 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1-2 μg/IP Fig 1
ELISA 1:100 Fig 2
Western Blotting 1:1,000 Fig 3
Immunofluorescence 1:1,000 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    H4pan Antibody ChIP Grade

    Figure 1 ChIP results obtained with the Diagenode antibody directed against H4
    ChIP assays were performed using human HeLa cells, the Diagenode antibody against H4 (Cat. No. C15410156) and optimized PCR primer pairs for qPCR. ChIP was performed with the “Auto Histone ChIP-seq” kit (Cat. No. C01010022), using sheared chromatin from 1 million cells on the IP-Star Compact automated system. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and c-fos genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    H4pan Antibody ELISA validation

    Figure 2 Determination of the titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H4 (Cat. No. C15410156) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:3,000.

    H4pan Antibody validated in Western Blot

    Figure 3 Western blot analysis using the Diagenode antibody directed against H4
    Western blot was performed on whole cell extracts (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Diagenode antibody against H4 (Cat. No. C15410156). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    H4pan Antibody validated in Immunofluorescence

    Figure 4. Immunofluorescence using the Diagenode antibody directed against H4
    HeLa cells were stained with the Diagenode antibody against H4 (Cat. No. C15410156) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the H4 antibody (middle) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Target Description

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a central role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes.

  •  应用
    Enzyme-linked immunosorbent assay. Read more
    ChIP-qPCR (ab)
    Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  文档
    Datasheet H4pan pAb-156-050 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    SDS C15410156 H4pan Antibody US en Download
    SDS C15410156 H4pan Antibody GB en Download
    SDS C15410156 H4pan Antibody ES es Download
    SDS C15410156 H4pan Antibody BE fr Download
    SDS C15410156 H4pan Antibody FR fr Download
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    SDS C15410156 H4pan Antibody JP ja Download
    SDS C15410156 H4pan Antibody DE de Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4pan polyclonal antibody (Diagenode Cat# C15410156-50 Lot# A1217P). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    HIRA supports hepatitis B virus minichromosome establishment andtranscriptional activity in infected hepatocytes.
    Locatelli M. et al.
    BACKGROUND \& AIMS: Upon Hepatitis B virus (HBV) infection, partially double stranded viral DNA converts into a covalently-closed-circular chromatinized episomal structure (cccDNA). This form represents the long-lived genomic reservoir responsible for viral persistence in the infected liver. While the involvemen...

    Kaposi’s Sarcoma-Associated Herpesvirus Processivity Factor,ORF59, Binds to Canonical and Linker Histones, and ItsCarboxy Terminus Is Dispensable for Viral DNA Synthesis
    Gutierrez IV et al.
    Kaposi’s sarcoma-associated herpesvirus (KSHV) is an oncogenic virus and the causative agent of potentially fatal malignancies. Lytic replication of KSHV is an essential part of the viral life cycle, allowing for virus dissemination within the infected host and shedding to infect naive hosts.

    Passaging Techniques and ROCK Inhibitor Exert Reversible Effects on Morphology and Pluripotency Marker Gene Expression of Human Embryonic Stem Cell Lines.
    Holm F, Nikdin H, Kjartansdóttir KR, Gaudenzi G, Fried K, Aspenström P, Hermanson O, Bergström-Tengzelius R
    Human embryonic stem cells (hESCs) are known for their potential usage in regenerative medicine, but also for handling sensitivity. Much effort has been put into optimizing the culture methods of hESCs. It has been shown that the use of Rho-associated coiled-coil kinase inhibitor (ROCKi) decreases the cellular stres...

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