H3T3pK4ac polyclonal antibody

50 μg
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (ac Lys4, p Thr3), using a KLH-conjugated synthetic peptide.

Concentration0.57 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells
Immunohistochemistry 1:50
Immunofluorescence 1:100 Figure 1
Western Blotting 1:500 Figure 2
Dot Blotting 1:1,000 Figure 3
  • Validation Data

    Immunofluorescence results

    Figure 1. H3T3pK4ac antibody Immunofluorescence results
    Immunofluorescence Microscopy of H3T3pK4ac antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3T3pK4ac is nuclear and chromosomal. Staining: H3T3pK4ac is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).

    Western blot results

    Figure 2. H3T3pK4ac antibody Western blot results
    Western Blot of H3T3pK4ac antibody. Lane 1: HeLa histone extracts. Lane 2. NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Dot blot results

    Figure 3. H3T3pK4ac antibody Dot blot results
    Dot Blot of H3T3pK4ac antibody. Lane 1: T3p. Lane 2: T3pK4me1. Lane 3: T3pK4me2. Lane 4: T3pK4ac. Lane 5: T3pR2me2s. Lane 6: K4 unmodified. Lane 7: K4me1. Lane 8: K4me2. Lane 9: K4me3. Lane 10: K4ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1,000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  •  应用
    Immunohistochemistry Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Dot blotting Read more
  •  文档
    Datasheet H3T3pK4ac C15410279 DATASHEET
    Datasheet description
  •  出版物

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