ctr9/PAF1 polyclonal antibody

100 µl
  Bulk order

Alternative names: SH2BP1, TSBP, p150, p150TSP

Polyclonal antibody raised in rabbit against mouse ctr9/PAF1 (ctr9, Paf1/RNA polymerase II complex component), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the N-terminal part of the protein.

Concentrationnot determined
Species reactivityMouse
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:500 Fig 1
Western Blotting 1:500 Fig 2 (1)
  • Validation Data


    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse ctr9/PAF1 (Cat. No. CS-112-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:4,600.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against ctr9/PAF1 (1)
    Western blot was performed on nuclear extracts and whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse ctr9/PAF1 (Cat. No. CS-112-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 133 kDa) is indicated on the right.

  •  应用
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  文档
    Datasheet ctr9-PAF1 CS-112-100 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    ctr9/PAF1 Antibody SDS GB en Download
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    ctr9/PAF1 Antibody SDS FR fr Download
    ctr9/PAF1 Antibody SDS ES es Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ctr9/PAF1 polyclonal antibody (Diagenode Cat# C15310112 Lot# A365-004 ). Click here to copy to clipboard.

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