Diagenode

Premium RRBS kit V2 - Spike-in controls analysis to estimate bisulfite conversion efficiency

This document describes how to process demultiplexed RRBS V2 sequencing data to extract the information from the spike-in controls included in the Premium RRBS Kit v2 in order to estimate the bisulfite conversion efficiency.

Sequences of the methylated and unmethylated spike-in controls, as well as the positions of the unmethylated cytosines present in the methylated spike-in control, can be downloaded from the ‘Documents’ section of the Premium RRBS Kit v2 page https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24:

  • RRBS_methylated_control.fa: the sequence of the methylated spike-in control in FASTA format
  • RRBS_unmethylated_control.fa: the sequence of the unmethylated spike-in control in FASTA format
  • RRBS_control_unmC.bed: the positions of the unmethylated cytosines in the sequence of the methylated control in BED format

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