Diagenodeの新しいRNAシークエンシングソリューションは、革新的な「テーリングとスイッチによる捕捉と増幅」（CATS）を利用し、RNAの低入力量から次世代シークエンシングのためのDNAライブラリーを調製するライゲーションフリーの方法です。
さまざまな転写産物の検出
10pgまでの超低入力で優れた再現性
時間短縮、優れた再生性をもっとシンプルにできるCATS mRNA-seqについて更に詳しくはこちら
血しょう由来低分子RNAサンプルにおけるCATS small RNA-seqに関してのお客様の声はこちら
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Transcripts detected in two technical replicates of CATS v2 total RNA-seq libraries. TPM≥2
Input: 1 ng rRNA(-) RNA from human universal total RNA (Agilent, 740000).
All detected transcripts in CATS v2 mRNA-seq Kit vs NEBNext Ultra I directional library preparation Kit. No threshold has been set for the analysis.
Input: 1 ng poly(A) RNA from human universal total RNA (Agilent, 740000). 36,245 transcripts found in both libraries.
Biotypes* represented in the libraries CATS v2 mRNA-seq Kit vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. Input: 1 ng, 10 ng poly(A) RNA from human universal total RNA (Agilent, 740000). * < 0.04% of total detected transcripts at this threshold is rRNA
Heatmap clustering of the top 50 differentially expressed microRNAs(miRNAs) after library preparation:
Prior library preparation, exosomes were isolated with Diagenode exosome capture solutions Exosomes
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