Diagenode

タンパク質分析

タンパク質抽出

Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Diagenode's Bioruptor® deliver high quality protein.

Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).

Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).

タンパク質抽出サービスの推奨・関連製品

Cat. No.ProductFormatPrice
B01060010 Picoruptor ® Sonicator
3種類のPicoruptorパッケージ(オールインワン)もお求め頂けます B01060011 - Picoruptor with tube holder and adaptor for 1.5ml tubes B01060012 - Picoruptor with tube holder and adap...
1 unit
C30010010-50 1.5 ml Bioruptor® Plus TPX microtubes
TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultra...
50 pc $45.00
B01020001 Bioruptor® Plus sonication device
The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (wate...
1 unit
C20000021 Protein Extraction beads
Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein...
20 g $85.00
C20000020 Protein Extraction kit for Bioruptor® Plus
Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein...
50 rxns $390.00

関連するプロトコル

Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico
Protein extraction from tissues and cultured cells is the first step for many biochemical and ana...
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TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein com...
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Activity Test - Procedure for immunoprecipitation of native chromatin proteins
Proteins play role in almost all cellular processes, and the study of their activity is crucial f...
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Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®
The regular protocol for the extraction of histone requires an acid extraction, making impossible...
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Mass-Spectrometry: procedure for shotgun proteomics
Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Sh...
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Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis
Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck ...
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iST Sample Preparation with the Bioruptor®
The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e...
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