Diagenode

タンパク質分析

タンパク質抽出

Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Diagenode's Bioruptor® deliver high quality protein.

Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).

Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).

タンパク質抽出サービスの推奨・関連製品

Cat. No.ProductFormatPrice
B01080010 Picoruptor 2 sonication device
Coming Soon to Japan! Since 2004, Diagenode has accumulated shearing expertise to design the Picoruptor and guarantee the best experience with the sample pr...
1 unit
B01020001 Bioruptor® Plus sonication device
The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (wate...
1 unit
C30010010-50 1.5 ml Bioruptor® Plus TPX microtubes
TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultra...
50 pc $45.00
B01200045 iST cartridge holder for the Picoruptor (PreOmics)
The iST cartridge holder has been especially designed for being used with the Picoruptor®  sonication device and with the PreOmics iST sample prepar...
1 pack $1,500.00
B01060010 Picoruptor Sonicator
Check our new Picoruptor shearing device here. Picoruptorは、微小容量の5μlから最大2mlの大容量のせん断に最適化されたオールインワンせん断システムで、断片化における最新の革新技術です。内蔵の冷却システム(Picoruptor Cooler)に...
1 unit
C20000021 Protein Extraction beads
Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein...
20 g $90.00
C20000020 Protein Extraction kit for Bioruptor® Plus
Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein...
50 rxns $395.00
B01201140 Tube holder for 1.5 ml tubes - Picoruptor
The tube holder for 1.5 ml tubes has been especially designed for being used with the Picoruptor sonication device. It allows for reproducible sonication of ...
1 unit

関連するプロトコル

Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico
Protein extraction from tissues and cultured cells is the first step for many biochemical and ana...
ダウンロード
TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein com...
ダウンロード
Activity Test - Procedure for immunoprecipitation of native chromatin proteins
Proteins play role in almost all cellular processes, and the study of their activity is crucial f...
ダウンロード
Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®
The regular protocol for the extraction of histone requires an acid extraction, making impossible...
ダウンロード
Mass-Spectrometry: procedure for shotgun proteomics
Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Sh...
ダウンロード
Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis
Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck ...
ダウンロード
iST Sample Preparation with the Bioruptor®
The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e...
ダウンロード

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  • EpiChrom
    Umea Sweden
    Feb 27-Feb 28, 2020
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