Mass Spectrometry

16 publications found.

A set of novel automated quantitative microproteomics protocols for small sample amounts and its application to kidney tissue substructures
Published: November, 2016

Abstract: Here we assessed the ability of an automated sample preparation device equipped with disposable microcolumns to prepare mass-limited samples for high-sensitivity quantitative proteomics, using both label-free and isobaric labeling approaches. Firs...
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Phosphorylation of TET proteins is regulated via O-GlcNAcylation by the glycosyltransferase OGT.
Published: January, 2015

Abstract: TET proteins oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC) and 5-carboxylcytosine (caC) and thus provide a possible means for active DNA demethylation in mammals. Although their catalytic mechanism is well c...
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Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster.
Published: November, 2014

Abstract: The ecdysone signaling pathway plays a major role in various developmental transitions in insects. Recent advances in the understanding of ecdysone action have relied to a large extent on the application of molecular genetic tools in Drosophila. H...
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Serine Deprivation Enhances Anti-neoplastic Activity of Biguanides.
Published: November, 2014

Abstract: Metformin, a biguanide widely used in the treatment of type II diabetes, clearly exhibits anti-neoplastic activity in experimental models and has been reported to reduce cancer incidence in diabetics. . There are ongoing clinical trials to evaluat...
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Precision microbiome reconstitution restores bile acid mediated resistance to Clostridium difficile.
Published: October, 2014

Abstract: The gastrointestinal tracts of mammals are colonized by hundreds of microbial species that contribute to health, including colonization resistance against intestinal pathogens. Many antibiotics destroy intestinal microbial communities and increase...
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Proteomics-Based Identification of Differentially Abundant Proteins from Human Keratinocytes Exposed to Arsenic Trioxide
Published: June, 2014

Abstract: Introduction: Arsenic i s a widely distributed environmental toxicant that can cause multi-tissue pathologies. Proteomic assays allow for the identification of biological processes modulated by arsenic in diverse tissue types. Method: The altered ...
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Sequestration by IFIT1 impairs translation of 2'O-unmethylated capped RNA.
Published: October, 2013

Abstract: Viruses that generate capped RNA lacking 2'O methylation on the first ribose are severely affected by the antiviral activity of Type I interferons. We used proteome-wide affinity purification coupled to mass spectrometry to identify human and mous...
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Mutant IDH1 promotes leukemogenesis in vivo and can be specifically targeted in human AML.
Published: August, 2013

Abstract: Mutations in the metabolic enzymes isocitrate dehydrogenase 1 (IDH1) and 2 (IDH2) are frequently found in glioma, acute myeloid leukemia (AML), melanoma, thyroid cancer and chondrosarcoma patients. Mutant IDH produces 2-hydroxyglutarate (2HG), whi...
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The complete targeted profile of the organic acid intermediates of the citric acid cycle using a single stable isotope dilution analysis, sodium borodeuteride reduction and selected ion monitoring GC/MS
Published: April, 2013

Abstract: The quantitative profiling of the organic acid intermediates of the citric acid cycle (CAC) presents a challenge due to the lack of commercially available internal standards for all of the organic acid intermediates. We developed an analytical met...
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A Quantitative Proteomic Workflow for Characterization of Frozen Clinical Biopsies: Laser Capture Microdissection Coupled with Label-Free Mass Spectrometry
Published: September, 2012

Abstract: This paper describes a simple, highly efficient and robust proteomic workflow for routine liquid-chromatography tandem mass spectrometry analysis of Laser Microdissection Pressure Catapulting (LMPC) isolates. Highly efficient protein recovery was ...
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Characterization of glomerular diseases using proteomic analysis of laser capture microdissected glomeruli.
Published: May, 2012

Abstract: The application of molecular techniques to characterize clinical kidney biopsies has the potential to provide insights into glomerular diseases that cannot be revealed by traditional renal pathology. The present work is a proof-of-concept approach...
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The oxidized thiol proteome in fission yeast--optimization of an ICAT-based method to identify H2O2-oxidized proteins.
Published: October, 2011

Abstract: Major intracellular disulfide formation is prevented in the cytosol by potent reducing systems. However, protein thiols can be oxidized as a consequence of redox-mediated physiological reactions or due to the unwanted toxicity of reactive oxygen s...
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Simultaneous quantitative analysis of N-acylethanolamides in clinical samples.
Published: December, 2009

Abstract: A simple and rapid analytical method is described for the simultaneous quantitative analysis of three different N-acylethanolamides in human biological samples: anandamide (AEA), oleoylethanolamide (OEA), and palmitoylethanolamide (PEA). The metho...
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Gel-based and gel-free proteomic analysis of Nicotiana tabacum trichomes identifies proteins involved in secondary metabolism and in the (a)biotic stress response

Abstract: Nicotiana tabacum leaves are covered by trichomes involved in the secretion of large amounts of secondary metabolites, some of which play a major role in plant defense. However, little is known about the metabolic pathways that operate in these st...
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Step-by-step preparation of proteins for mass spectrometric analysis.

Abstract: Nowadays, identification of proteins from biological samples by mass spectrometry is widely used. In principle there are two scenarios. Proteins are pre-fractionated in some way, e.g. by gel electrophoresis or are analyzed as complex mixture (shot...
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Intracellular MMP-2 Activity in Skeletal Muscle Is Associated With Type II Fibers.

Abstract: Matrix metalloproteinase 2 (MMP-2) is a proteolytic enzyme implicated in motility, differentiation, and regeneration of skeletal muscle fibers through processing of extracellular substrates. Although MMP-2 has been found to be localized intracellu...
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