Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. Published: August, 2015 Abstract:
CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering. However, the delivery of Cas9 and synthesis of guide RNA (gRNA) remain as steps that can limit overa... Read more »
Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Published: March, 2015 Abstract:
Genetic screens are powerful tools for identifying genes responsible for diverse phenotypes. Here we describe a genome-wide CRISPR/Cas9-mediated loss-of-function screen in tumor growth and metastasis. We mutagenized a non-metastatic mouse cancer c... Read more »
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