Diagenode

Characterization of eight polymorphic loci for Maasai giraffe (Giraffa camelopardalis tippelskirchi) using non-invasive genetic samples


Crowhurst RS, Mullins TD, Mutayoba BM, Epps CW.

We used Illumina sequencing to develop eight novel microsatellite loci for Maasai giraffe (Giraffa camelopardalis tippelskirchi), and screened them using fecal DNA. Genetic diversity was assessed for 40 individuals from the Katavi National Park/Rukwa Game Reserve ecosystem in Tanzania. The number of alleles per locus ranged from 2 to 14 (mean = 6.2) and mean expected heterozygosity was moderate (range 0.025–0.838, mean = 0.527). These markers were successfully tested using degraded DNA and may be useful for future studies of giraffe, especially those that use non-invasive sampling techniques.

Tags
DNA shearing
Bioruptor

Share this article

Published
August, 2012

Source

Events

 See all events

Twitter feed

News

 See all news