Diagenode

Incretin drugs effect on epigenetic machinery: New potential therapeuticimplications in preventing vascular diabetic complications.


Scisciola, Lucia and Rizzo, Maria Rosaria and Cataldo, Vittoria andFontanella, Rosaria Anna and Balestrieri, Maria Luisa and D'Onofrio, Nunziaand Marfella, Raffaele and Paolisso, Giuseppe and Barbieri, Michelangela

The effect of GLP-1R agonists on DNA methylation levels of NF-κB and SOD2 genes in human aortic endothelial cells exposed to high glucose and in diabetic patients treated and not with incretin-based drugs, was evaluated. Methylation levels, mRNA and protein expression of NF-κB and SOD2 genes were measured in human endothelial cells exposed to high glucose for 7 days and treated with GLP-1R agonists. Methylation status of NF-κB and SOD2 promoter was also analyzed in 128 diabetics and 116 nondiabetics and correlated with intima media thickness (ITM), an early marker of atherosclerotic process. Cells exposed to high glucose showed lower NF-κB and SOD2 methylation levels, increased NF-κB and reduced SOD2 expression compared to normal glucose cells. Co-treatment with GLP-1 agonists prevented methylation and genes expression changes induced by high glucose. Both high glucose and incretins exposure increased DNA methyltransferases and demethylases levels. In diabetics, incretin treatment resulted a significant predictor of NF-κB DNA methylation, independently of age, sex, body mass index (BMI), glucose and plasma lipid levels. NF-κB DNA methylation inversely correlated with IMT after adjusting for multiple covariates. Our results firstly provide new evidences of an additional mechanism by which incretin drugs could prevent vascular diabetic complications.

Tags
Antibody

Share this article

Published
December, 2020

Source

Products used in this publication

  • Antibody ChIP icon
    C15410255-100
    TET2 Antibody - ChIP Grade

Events

  • Virtual ChIL-seq webinar
    Diagenode
    Dec 8, 2021
 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics