Diagenode

m6A-dependent maternal mRNA clearance facilitates zebrafish maternal-to-zygotic transition


Boxuan Simen Zhao, Xiao Wang, Alana V. Beadell, Zhike Lu, Hailing Shi, Adam Kuuspalu, Robert K. Ho & Chuan He

The maternal-to-zygotic transition (MZT) is one of the most profound and tightly orchestrated processes during the early life of embryos, yet factors that shape the temporal pattern of vertebrate MZT are largely unknown. Here we show that over one-third of zebrafish maternal messenger RNAs (mRNAs) can be N6-methyladenosine (m6A) modified, and the clearance of these maternal mRNAs is facilitated by an m6A-binding protein, Ythdf2. Removal of Ythdf2 in zebrafish embryos decelerates the decay of m6A-modified maternal mRNAs and impedes zygotic genome activation. These embryos fail to initiate timely MZT, undergo cell-cycle pause, and remain developmentally delayed throughout larval life. Our study reveals m6A-dependent RNA decay as a previously unidentified maternally driven mechanism that regulates maternal mRNA clearance during zebrafish MZT, highlighting the critical role of m6A mRNA methylation in transcriptome switching and animal development.

Tags
Bioruptor

Share this article

Published
December, 2016

Source

Related product

  • Bioruptor-pico-next-gen-sequencing
    B01060001
    Bioruptor® Pico sonication device

Events

  • "Molecular Biosystems" Conference on Eukaryotic Gene Regulation and Functional Genomics
    Puerto Varas, Región de Los Lagos, Chile
    Sep 23-Sep 26, 2017
  • The 76th Annual Meeting of the Japanese Cancer Association
    Tokyo, Japan
    Sep 28-Sep 30, 2017
 See all events

Twitter feed

News

 See all news