Genome-wide DNA methylation profile of developing deciduous tooth germ in miniature pigs
Su Y, Fan Z, Wu X, Li Y, Wang F, Zhang C, Wang J, Du J, Wang S
DNAmethylation is an important epigenetic modification critical to the regulation of gene expression during development. To date, little is known about the role of DNAmethylation in tooth development in large animal models. Thus, we carried out a comparative genomic analysis of genome-wideDNAmethylation profiles in E50 and E60 toothgerm from miniaturepigs using methylated DNA immunoprecipitation-sequencing (MeDIP-seq).
We observed different DNAmethylation patterns during the different developmental stages of pig toothgerm. A total of 2469 differentially methylated genes were identified. Functional analysis identified several signaling pathways and 104 genes that may be potential key regulators of pig tooth development from E50 to E60.
The present study provided a comprehensive analysis of the global DNAmethylation pattern of toothgerm in miniaturepigs and identified candidate genes that potentially regulate tooth development from E50 to E60.