Diagenode

R432 is a key residue for the multiple functions of Ndt80p in Candida albicans.


Yang YL, Wang CW, Leaw SN, Chang TP, Wang IC, Chen CG, Fan JC, Tseng KY, Huang SH, Chen CY, Hsiao TY, Hsiung CA, Chen CT, Hsiao CD, Lo HJ

Ndt80p is an important transcription modulator to various stress-response genes in Candida albicans, the most common human fungal pathogen in systemic infections. We found that Ndt80p directly regulated its target genes, such as YHB1, via the mid-sporulation element (MSE). Furthermore, the ndt80 ( R432A ) allele, with a reduced capability to bind MSE, failed to complement the defects caused by null mutations of NDT80. Thus, the R432 residue in the Ndt80p DNA-binding domain is involved in all tested functions, including cell separation, drug resistance, nitric oxide inactivation, germ tube formation, hyphal growth, and virulence. Hence, the importance of the R432 residue suggests a novel approach for designing new antifungal drugs by blocking the interaction between Ndt80p and its targets.

Tags
Bioruptor
Chromatin Shearing
ChIP-qPCR

Share this article

Published
October, 2011

Source

Events

  • AACR 2024
    San Diego, California, USA
    Apr 5-Apr 10, 2024
 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics