Location Analysis and Expression Profiling Using Next-Generation Sequencing for Research in Neurodegenerative Diseases

Gotovac K, Borovečki F, Habek M

Neurodegenerative diseases carry a huge burden in terms of human suffering and economic cost. In spite of advances in the field of neurology, there is still no effective cure. A combination of novel genomic approaches and techniques such as chromatin immunoprecipitation sequencing (ChIP-seq) and RNA-seq that enable high-quality data could provide better understanding of transcriptional and epigenetic signatures useful for both biomarker development and drug discovery in neurodegenerative diseases. This chapter provides detailed protocols of the different steps required to generate a successful ChIP-seq and RNA-seq library. ChIP-seq protocol starts with cell collection and fixation, chromatin preparation, immunoprecipitation, and finally library preparation. RNA-seq protocol described in this chapter starts with the isolation of peripheral blood mononuclear cells (PBMCs), RNA isolation from cells, and library preparation. The ChIP-seq protocol is optimized for human neuroglioma cells (H4), and RNA-seq protocol is optimized for PBMCs, but both protocols can be adapted to different cell types with minor modifications. The obtained libraries are suitable for sequencing on Illumina GAIIx platform.

Chromatin Shearing

Share this article



  • IHEC Annual meeting 2022
    Quebec, Canada
    Oct 4-Oct 7, 2022
  • Danish RNA Society's 7th Annual Meeting on RNA
    Aarhus, Denmark
    Oct 11, 2022
  • ASHG 2022
    Los Angeles, California, USA
    Oct 25-Oct 29, 2022
  • International Conference on Epigenetics and Bioengineering 2022
    Houston, Texas, USA
    Oct 27-Oct 29, 2022
  • Long-Read Sequencing Meeting Uppsala LRUA22 Meeting
    Uppsala, Sweden
    Oct 31-Nov 2, 2022
  • Association for Molecular Pathology 2022
    Phoenix, Arizona, USA
    Nov 1-Nov 5, 2022
 See all events


 See all news

The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics