Diagenode

Genome-Wide Characterization of Light-Regulated Genes in Neurospora crassa.


Wu C, Yang F, Smith KM, Peterson M, Dekhang R, Zhang Y, Zucker J, Bredeweg EL, Mallappa C, Zhou X, Lyubetskaya A, Townsend JP, Galagan JE, Freitag M, Dunlap JC, Bell-Pedersen D, Sachs MS

The filamentous fungus Neurospora crassa responds to light in complex ways. To thoroughly study the transcriptional response of this organism to light, RNA-seq was used to analyze capped and polyadenylated mRNA prepared from mycelium grown for 24 hr in the dark and then exposed to light for 0 (control) 15, 60, 120 and 240 min. More than three quarters of all defined protein coding genes (79%) were expressed in these cells. The increased sensitivity of RNA-seq compared to previous microarray studies revealed that the RNA levels for 31% of expressed genes were affected 2-fold or more by exposure to light. Additionally, a large class of mRNAs, enriched for transcripts specifying products involved in rRNA metabolism, showed decreased expression in response to light, indicating a heretofore undocumented effect of light on this pathway. Based on measured changes in mRNA levels, light generally increases cellular metabolism, and at the same time causes significant oxidative stress to the organism. To deal with this stress, protective photopigments are made, antioxidants are produced and genes involved in ribosome biogenesis are transiently repressed.

Tags
DNA shearing
Bioruptor

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Published
July, 2014

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