Diagenode

Genome-wide profiling of transcription factor binding and epigenetic marks in adipocytes by ChIP-seq.


Nielsen R, Mandrup S

The recent advances in high-throughput sequencing combined with various other technologies have allowed detailed and genome-wide insight into the transcriptional networks that control adipogenesis. Chromatin immunoprecipitation (ChIP) combined with high-throughput sequencing (ChIP-seq) is one of the most widely used of these technologies. Using these methods, association of transcription factors, cofactors, and epigenetic marks can be mapped to DNA in a genome-wide manner. Here, we provide a detailed protocol for performing ChIP-seq analyses in preadipocytes and adipocytes. We have focused mainly on critical points, limitations of the assay, and quality controls required in order to obtain reproducible ChIP-seq data.

Tags
Bioruptor
Chromatin Shearing
ChIP-seq

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Published
January, 2014

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