Diagenode

Experimental Study on Malignant Transformation of Human Bronchial Epithelial Cells Induced by Glycidyl Methacrylate and Analysis on its Methylation


WANG AN, WANG QK, YANG M, HU J, DONG L,XU JN

Objective To establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA) and define the different methylation genes at different stages. Methods DNA was extracted at different 16HBE malignant phases and changes of genes DNA methylation at different stages were detected using Methylation chip of “NimbleGen HG18 CpG Promoter Microarray Methylation”. Methylation-specific PCR (MSP) was used to observe the methylation status of some genes, and then compared with the control groups. Results The result showed that GMA induced 16HBE morphorlogical transformation at the dose of 8μg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes in metaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not in metaphase and anaphase; 272 genes in metaphase but not in protophase and anaphase; 683 genes in anaphase but not in metaphase and protophase; 73 genes in protophase and metaphase but not in anaphase; 67 genes in protophase and anaphase but not in metaphase; 59 genes in metaphase and anaphase but not in protophase. Conclusion The pattern of DNA methylation could change in the process of 16HBE induced by GMA.

Tags
DNA shearing
Bioruptor
Antibody

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