By using the True μChIP kit, highly specific DNA pull-down with unsurpassedsignal/noise ratio can be achieved using limited number of cells. Through the incorporation of unique features such as optimized fixation, binding and wash buffers, the Diagenode DiaMag magnetic beads and highly validated Diagenode ChIP-seq grade antibodies, superior ChIP-seq results can be achieved using as few as 10,000 cells.
Combining the MicroPlex Library Preparation kit with the high-quality ChIP DNA obtained from the True μChIP kit allows fast and easy ChIP-Seq library preparation for multiplex NGS analysis from picogram to nanogram input levels.
The True μChIP & MicroPlex Library Preparation kits have been thoroughly validated using several histone marks on Illumina sequencers. The results of our validation studies demonstrate a perfect match between the Top 40% of True MicroChIP peaks and the reference dataset from the Broad Institute.
In addition, the True μChIP kit has been validated with the IP-Star® Compact automated platform.