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Explore Diagenode’s publication database. Learn how our products are used in diverse fields of research.

1474 publications found×
The chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 is essential for H3K27me3 binding and function during Arabidopsis development.
Published: January, 2009
Abstract: Polycomb group (PcG) proteins are essential to maintain gene expression patterns during development. Transcriptional repression by PcG proteins involves trimethylation of H3K27 (H3K27me3) by Polycomb Repressive Complex 2 (PRC2) in animals and plan...
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Antibody LowCell ChIP kit
TIPT2 and geminin interact with basal transcription factors to synergize in transcriptional regulation.
Published: January, 2009
Abstract: BACKGROUND: The re-replication inhibitor Geminin binds to several transcription factors including homeodomain proteins, and to members of the polycomb and the SWI/SNF complexes. RESULTS: Here we describe the TATA-binding protein-like factor-intera...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody
Epigenetic engineering of ribosomal RNA genes enhances protein production.
Published: January, 2009
Abstract: Selection of mammalian high-producer cell lines remains a major challenge for the biopharmaceutical manufacturing industry. Ribosomal RNA (rRNA) genes encode the major component of the ribosome but many rRNA gene copies are not transcribed due to ...
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Antibody Master Mix
Methylated DNA immunoprecipitation and microarray-based analysis: detection of DNA methylation in breast cancer cell lines.
Published: January, 2009
Abstract: The methylated DNA immunoprecipitation microarray (MeDIP-chip) is a genome-wide, high-resolution approach to detect DNA methylation in whole genome or CpG (cytosine base followed by a guanine base) islands. The method utilizes anti-methylcytosine ...
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DNA shearing Bioruptor Antibody
Remodeling of the chromatin structure of the facioscapulohumeral muscular dystrophy (FSHD) locus and upregulation of FSHD-related gene 1 (FRG1) expression during human myogenic differentiation.
Published: January, 2009
Abstract: BACKGROUND: Facioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominant neuromuscular disorder associated with the partial deletion of integral numbers of 3.3 kb D4Z4 DNA repeats within the subtelomere of chromosome 4q. A number of cand...
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Antibody
Optimization of experimental design parameters for high-throughput chromatin immunoprecipitation studies.
Published: December, 2008
Abstract: High-throughput, microarray-based chromatin immunoprecipitation (ChIP-chip) technology allows in vivo elucidation of transcriptional networks. However this complex is not yet readily accessible, in part because its many parameters have not been sy...
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Bioruptor Chromatin Shearing ChIP-qPCR
NF-Y substitutes H2A-H2B on active cell-cycle promoters: recruitment of CoREST-KDM1 and fine-tuning of H3 methylations.
Published: November, 2008
Abstract: The CCAAT box is a frequent promoter element, as illustrated by bioinformatic analysis, and it is bound by NF-Y, a trimer with H2A-H2B-like subunits. We developed a MNase I-based ChIP protocol on homogeneous cell populations to study cell-cycle pr...
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Clozapine and sulpiride but not haloperidol or olanzapine activate brain DNA demethylation
Published: September, 2008
Abstract: Cortical GABAergic dysfunction, a hallmark of both schizophrenia (SZ) and bipolar (BP) disorder pathophysiologies may relate to the hypermethylation of GABAergic gene promoters (i.e., reelin and GAD67). Benefits elicited by a combination of atypic...
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Inactivation of a Human Kinetochore by Specific Targeting of Chromatin Modifiers
Published: April, 2008
Abstract: We have used a human artificial chromosome (HAC) to manipulate the epigenetic state of chromatin within an active kinetochore. The HAC has a dimeric α-satellite repeat containing one natural monomer with a CENP-B binding site, and one completely a...
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DNA shearing Antibody
Identification of CIITA regulated genetic module dedicated for antigen presentation.
Published: April, 2008
Abstract: The class II trans-activator CIITA is a transcriptional co-activator required for the expression of Major Histocompatibility Complex (MHC) genes. Although the latter function is well established, the global target-gene specificity of CIITA had not...
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The Friedreich ataxia GAA repeat expansion mutation induces comparable epigenetic changes in human and transgenic mouse brain and heart tissues.
Published: March, 2008
Abstract: Friedreich ataxia (FRDA) is caused by a homozygous GAA repeat expansion mutation within intron 1 of the FXN gene, leading to reduced expression of frataxin protein. Evidence suggests that the mutation may induce epigenetic changes and heterochroma...
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MicroChIP--a rapid micro chromatin immunoprecipitation assay for small cell samples and biopsies.
Published: February, 2008
Abstract: Chromatin immunoprecipitation (ChIP) is a powerful technique for studying protein-DNA interactions. Drawbacks of current ChIP assays however are a requirement for large cell numbers, which limits applicability of ChIP to rare cell samples, and/or ...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody LowCell ChIP kit
A rapid micro chromatin immunoprecipitation assay (microChIP).
Published: January, 2008
Abstract: Interactions of proteins with DNA mediate many critical nuclear functions. Chromatin immunoprecipitation (ChIP) is a robust technique for studying protein-DNA interactions. Current ChIP assays, however, either require large cell numbers, which pre...
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Antibody
Epigenetic regulation (DNA methylation, histone modifications) of the 11p15 mucin genes (MUC2, MUC5AC, MUC5B, MUC6) in epithelial cancer cells.
Published: October, 2007
Abstract: The human genes MUC2, MUC5AC, MUC5B and MUC6 are clustered on chromosome 11 and encode large secreted gel-forming mucins. The frequent occurrence of their silencing in cancers and the GC-rich structure of their promoters led us to study the influe...
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Mallory body formation is associated with epigenetic phenotypic change in hepatocytes in vivo.
Published: October, 2007
Abstract: Microarrays were done on the livers of mice fed DDC for 10 weeks, withdrawn 1 month (DDC primed livers) and refed 6 days, and compared with mice fed the control diet. The expression of a large number of genes changed when DDC was fed or refed. A V...
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Identification of novel functional TBP-binding sites and general factor repertoires
Published: February, 2007
Abstract: Our current knowledge of the general factor requirement in transcription by the three mammalian RNA polymerases is based on a small number of model promoters. Here, we present a comprehensive chromatin immunoprecipitation (ChIP)-on-chip analysis f...
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Reciprocal regulation of p63 by C/EBP delta in human keratinocytes.
Published: January, 2007
Abstract: BACKGROUND: Genetic experiments have clarified that p63 is a key transcription factor governing the establishment and maintenance of multilayered epithelia. Key to our understanding of p63 strategy is the identification of target genes. We perfome...
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Using ChIP-Seq Technology to Generate High-Resolution Profiles of Histone Modifications
Abstract: The dynamic modification of DNA and histones plays a key role in transcriptional regulation through - altering the packaging of DNA and modifying the nucleosome surface. These chromatin states, also referred to as the epigenome, are distinctive fo...
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Bioruptor Chromatin Shearing ChIP-seq IP-Star Antibody
Role of transcriptional and post-transcriptional regulation of methionine adenosyltransferases in liver cancer progression
Abstract: Downregulation of liver-specific MAT1Agene, encoding S-adenosylmethionine (SAM) synthesizing isozymes MATI/III, and upregulation of widely expressedMAT2A, encoding MATII isozyme, known as MAT1A:MAT2A switch, occurs in hepatocellular carcinoma (HCC...
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Antibody
Analysis of active chromatin modifications in early mammalian embryos reveals uncoupling of H2A.Z acetylation and H3K36 trimethylation from embryonic genome activation
Abstract: Early embryonic development is characterized by dramatic changes in cell potency and chromatin organization. The role of histone variants in the context of chromatin remodeling during embryogenesis remains under investigated. In particular, the nu...
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Antibody H3K4me3 (C15410003)
Regulation of EP receptors in non-small cell lung cancer by epigenetic modifications.
Abstract: BACKGROUND: Cyclooxygenase (COX)-2 is frequently overexpressed in non-small cell lung cancer (NSCLC) and results in increased levels of prostaglandin E2 (PGE(2)), an important signalling molecule implicated in tumourigenesis. PGE(2) exerts its ef...
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Antibody
Whole genome methylation profiling by immunoprecipitation of methylated DNA
Abstract: I provide a protocol for DNA methylation profiling based on immunoprecipitation of methylated DNA using commercially available monoclonal antibodies that specifically recognize 5-methylcytosine. Quantification of the level of enrichment of the res...
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Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells.
Abstract: Methylated DNA immunoprecipitation (MeDIP) is an immunocapturing approach for unbiased enrichment of DNA that is methylated on cytosines. The principle is that genomic DNA is randomly sheared by sonication and immunoprecipitated with an antibody t...
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DNA shearing Bioruptor Antibody 5mC (C15200081)
Role of Transcriptional Corepressor CtBP1 in Prostate Cancer Progression
Abstract: Transcriptional repressors and corepressors play a critical role in cellular homeostasis and are frequently altered in cancer. C-terminal binding protein 1 (CtBP1), a transcriptional corepressor that regulates the expression of tumor suppressors a...
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Antibody
Chromatin immunoprecipitation analysis in filamentous fungi.
Abstract: Chromatin immunoprecipitation (ChIP) is used to map the interaction between proteins and DNA at a specific genomic locus in the living cell. The protein-DNA complexes are stabilized already in vivo by reversible crosslinking and the DNA is sheared...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody
A novel mechanism for CTCF in the epigenetic regulation of Bax in breast cancer cells.
Abstract: We previously reported the association of elevated levels of the multifunctional transcription factor, CCCTC binding factor (CTCF), in breast cancer cells with the specific anti-apoptotic function of CTCF. To understand the molecular mechanisms of...
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Antibody H3K9ac (C15200185)
Experimental Study on Malignant Transformation of Human Bronchial Epithelial Cells Induced by Glycidyl Methacrylate and Analysis on its Methylation
Abstract: Objective To establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA) and define the different methylation genes at different stages. Methods DNA was extracted at different 16...
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DNA shearing Bioruptor Antibody
Detection of DNA methylation by MeDIP and MBDCap assays: an overview of techniques.
Abstract: DNA methylation has been characterized as the representative example of epigenetic modifications and implicated in numerous biological processes, such as genomic imprinting and X chromosome inactivation. It primarily occurs at CpG dinucleotides in...
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DNA shearing Bioruptor Antibody
Epigenetic-Mediated Downregulation of μ-Protocadherin in Colorectal Tumours
Abstract: Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorect...
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Bioruptor Chromatin Shearing Antibody H3K4me3 (C15410003) Bioruptor Plus
Esculetin Downregulates the Expression of AML1-ETO and C-Kit in Kasumi-1 Cell Line by Decreasing Half-Life of mRNA
Abstract: One of the most frequent genetic aberrations in acute myeloid leukemia (AML) is chromosomal translocation between AML1/RUNX1 on chromosome 21 and ETO gene on chromosome 8 resulting in the expression of chimeric oncogene AML1-ETO. Although patients...
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Antibody

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