SAP30 Antibody - ChIP-seq Grade

50 µg
  Bulk order

Polyclonal antibody raised in rabbit against human SAP30 (Sin3-associated polypeptide, 30kDa), using the full length His-tagged protein.

Concentration2.0 µg/µl
Species reactivityHuman
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 2-5 μg/ChIP Fig 1, 2
Western Blotting 1:1;000 Fig 3

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    SAP30 Antibody ChIP-seq Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against SAP30
    ChIP assays were performed using HeLa cells, the Diagenode antibody against SAP30 (Cat. No. C15410036) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    SAP30 Antibody ChIP-seq Grade

    SAP30 Antibody for ChIP-seq

    SAP30 Antibody for ChIP-seq assay

    SAP30 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30
    ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 μg of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).

    SAP30 Antibody validated in Western blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30
    Western blot was performed on nuclear extracts from HeLa cells (20 μg) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

  • Target Description

    SAP30 (UniProtKB/Swiss-Prot entry O75446) is a component of the histone deacetylase complex, which also includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48. Histone acetylation and deacetylation play a key role in the regulation of eukaryotic gene expression. This complex only deacetylates core histone octamers, and is not able to deacetylate nucleosomal histones

  •  应用
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-seq (ab)
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    ChIP-qPCR (ab)
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  •  文档
    SAP30 polyclonal antibody - Classic DATASHEET
    SAP30 polyclonal antibody datasheet
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
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  •  出版物

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