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Mll2 polyclonal antibody

100 µl
  Bulk order

Alternative names: TRX2, WBP7, MLL4

Polyclonal antibody raised in rabbit against mouse Mll2 (Myeloid/lymphoid or mixed-lineage leukemia protein 2), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central and from the N-terminal part of the protein, respectively.

ConcentrationNot determined
Species reactivityMouse: positive. Other species: not tested.
PurityWhole antiserum from rabbit containing 0.05% azide.
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:200 - 1:2,000 Fig 1
Western Blotting 1:500 Fig 2, (1)
  • Validation Data


    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Mll2 (Cat. No. CS-099-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:32,500.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against Mll2
    Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Mll2 (Cat. No. CS-099-100), diluted 1:500 in BSA/PBS-Tween. The location of the N-terminal fragment of Mll2 is indicated on the right (270 kDa). Cells homozygous for the targeted conditional mll2 allele (ΔMll2) show a dramatic reduction of Mll2 protein after partial recombination, whereas Mll2 protein was detected in ΔMll1, ΔMll4 and WT E14Tg2a cells.

  •  应用
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  文档
    Datasheet Mll2 CS-099-100 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
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  •  出版物

    How to properly cite this product in your work

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